STUDIES OF THE ULTRASTRUCTURE AND RIBOSOMAL ARRANGEMENTS OF THE PLEUROPNEUMONIA-LIKE ORGANISM A5969
Open Access
- 1 April 1965
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 25 (1), 139-150
- https://doi.org/10.1083/jcb.25.1.139
Abstract
Thin-section electron microscopy, together with isolation of cellular organelles by differential centrifuga-tion and chemical analysis, has been used to investigate the ultrastructure of the avian pleuropneumonia-like organism-A5969. Each cell (approximate diameter 5500-A) was surrounded by a 150-A plasma membrane. In the center of the cell was an unbounded area, granular in appearance and containing the cell''s DNA. The periphery of the cell contained granules of several different sizes and densities. The most dense particles (150-A) corresponded to the 78S ribosomes. These particles exhibited two predominant arrangements: sometimes they showed cubic packing; most arrays, however, were consistent with cylindrical arrangements of approximately 50 particles. Bundles of up to 18 arrays were observed. Structured blebs have been found protruding from the surface of log phase cells.Keywords
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