Natural genetic resources of Arabidopsis thaliana reveal a high prevalence and unexpected phenotypic plasticity of RPW8‐mediated powdery mildew resistance
- 15 January 2008
- journal article
- Published by Wiley in New Phytologist
- Vol. 177 (3), 725-742
- https://doi.org/10.1111/j.1469-8137.2007.02339.x
Abstract
Here, an approach based on natural genetic variation was adopted to analyse powdery mildew resistance in Arabidopsis thaliana. Accessions resistant to multiple powdery mildew species were crossed with the susceptible Col-0 ecotype and inheritance of resistance was analysed. Histochemical staining was used to visualize archetypal plant defence responses such as callose deposition, hydrogen peroxide accumulation and host cell death in a subset of these ecotypes. In six accessions, resistance was likely of polygenic origin while 10 accessions exhibited evidence for a single recessively or semi-dominantly inherited resistance locus. Resistance in the latter accessions was mainly manifested at the terminal stage of the fungal life cycle by a failure of abundant conidiophore production. The resistance locus of several of these ecotypes was mapped to a genomic region containing the previously analysed atypical RPW8 powdery mildew resistance genes. Gene silencing revealed that members of the RPW8 locus were responsible for resistance to Golovinomyces orontii in seven accessions. These results suggest that broad-spectrum powdery mildew resistance in A. thaliana is predominantly of polygenic origin or based on RPW8 function. The findings shed new light on the natural variation of inheritance, phenotypic expression and pathogen range of RPW8-conditioned powdery mildew resistance.Keywords
This publication has 58 references indexed in Scilit:
- The Tomato NBARC-LRR Protein Prf Interacts with Pto Kinase in Vivo to Regulate Specific Plant ImmunityPlant Cell, 2006
- Genetic mechanisms and evolutionary significance of natural variation in ArabidopsisNature, 2006
- Mutations in PMR5 result in powdery mildew resistance and altered cell wall compositionThe Plant Journal, 2004
- EVOLUTIONARY POTENTIAL OF CHAMAECRISTA FASCICULATA IN RELATION TO CLIMATE CHANGE. I. CLINAL PATTERNS OF SELECTION ALONG AN ENVIRONMENTAL GRADIENT IN THE GREAT PLAINSEvolution, 2004
- REACTIVE OXYGEN SPECIES: Metabolism, Oxidative Stress, and Signal TransductionAnnual Review of Plant Biology, 2004
- Polygenic powdery mildew disease resistance in Arabidopsis thaliana: quantitative trait analysis of the accession Warschau‐1Plant Pathology, 2001
- Identification of two powdery mildew fungi, Oidium neolycopersici sp. nov. and O. lycopersici, infecting tomato in different parts of the worldMycological Research, 2001
- A new mathematical model for relative quantification in real-time RT-PCRNucleic Acids Research, 2001
- Comparison of Erysiphe cichoracearum and E. cruciferarum and a Survey of 360 Arabidopsis thaliana Accessions for Resistance to These Two Powdery Mildew PathogensMolecular Plant-Microbe Interactions®, 1999
- Genetic characterization of five powdery mildew disease resistance loci in Arabidopsis thalianaThe Plant Journal, 1996