Oligosaccharyltransferase directly binds to ribosome at a location near the translocon-binding site
Open Access
- 28 April 2009
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 106 (17), 6945-6949
- https://doi.org/10.1073/pnas.0812489106
Abstract
Oligosaccharyltransferase (OT) transfers high mannose-type glycans to the nascent polypeptides that are translated by the membrane-bound ribosome and translocated into the lumen of the endoplasmic reticulum through the Sec61 translocon complex. In this article, we show that purified ribosomes and OT can form a binary complex with a stoichiometry of ≈1 to 1 in the presence of detergent. We present evidence that OT may bind to the large ribosomal subunit near the site where nascent polypeptides exit. We further show that OT and the Sec61 complex can simultaneously bind to ribosomes in vitro. Based on existing data and our findings, we propose that cotranslational translocation and N-glycosylation of nascent polypeptides are mediated by a ternary supramolecular complex consisting of OT, the Sec61 complex, and ribosomes.Keywords
This publication has 40 references indexed in Scilit:
- Single Copies of Sec61 and TRAP Associate with a Nontranslating Mammalian RibosomeStructure, 2008
- Structure of the Oligosaccharyl Transferase Complex at 12 Å ResolutionStructure, 2008
- Dimeric organization of the yeast oligosaccharyl transferase complexProceedings of the National Academy of Sciences of the United States of America, 2006
- Architecture of the Ribosome–Channel Complex Derived from Native MembranesJournal of Molecular Biology, 2005
- X-ray structure of a protein-conducting channelNature, 2003
- Structure of the Mammalian Ribosome–Channel Complex at 17Å ResolutionJournal of Molecular Biology, 2002
- A comparison of the yeast and rabbit 80 S ribosome reveals the topology of the nascent chain exit tunnel, inter-subunit bridges and mammalian rRNA expansion segmentsJournal of Molecular Biology, 2000
- Protein translocation: Common themes from bacteria to manFEBS Letters, 1994
- Yeast Sec proteins interact with polypeptides traversing the endoplasmic reticulum membraneCell, 1992
- Co-ordinate synthesis of ribosomes and elongation factors in the liver of immature chicks following a metabolic shift upJournal of Molecular Biology, 1976