microRNA‐203 suppresses bladder cancer development by repressing bcl‐w expression
Open Access
- 4 January 2011
- journal article
- Published by Wiley in The FEBS Journal
- Vol. 278 (5), 786-792
- https://doi.org/10.1111/j.1742-4658.2010.07997.x
Abstract
It is increasingly clear that microRNAs (miRNAs) play an important role in many diseases, including tumorigenesis. However, the mechanisms by which miRNAs regulate bladder cancer development remain poorly understood. Here, we evaluated the expression of microRNA-203 (miR-203) in bladder cancer tissues using real-time PCR, and defined the target genes and biologically functional effect using luciferase reporter assay, flow cytometry and western blot analysis. We first verified that the expression of miR-203 was decreased in bladder cancer tissues. Moreover, ectopic expression of miR-203 promoted the apoptosis of human bladder cancer cell lines and inhibited cell proliferation, whereas its depletion increased cell growth. We further verified that miR-203 directly targeted 3′-untranslated region of the bcl-w gene, and decreased its expression in vitro and in vivo. Western blot analysis also showed that the expression level of miR-203 was negatively correlated with bcl-w level in tumor tissues. These data suggest an important role for miR-203 in the molecular etiology of bladder cancer and implicate the potential application of miR-203 in bladder cancer therapy.Keywords
This publication has 28 references indexed in Scilit:
- miR-29 Modulates Wnt Signaling in Human Osteoblasts through a Positive Feedback LoopJournal of Biological Chemistry, 2010
- Pelvic lymph nodes: distribution and nodal tumour burden of urothelial bladder cancerJournal of Clinical Pathology, 2010
- miR-145 and miR-133a function as tumour suppressors and directly regulate FSCN1 expression in bladder cancerBritish Journal of Cancer, 2010
- Predictive value of progression-related gene classifier in primary non-muscle invasive bladder cancerMolecular Cancer, 2010
- High frequency of tumor cells with nuclear Egr-1 protein expression in human bladder cancer is associated with disease progressionBMC Cancer, 2009
- MicroRNA Expression in Squamous Cell Carcinoma and Adenocarcinoma of the Esophagus: Associations with SurvivalClinical Cancer Research, 2009
- Outcome of recurrent and metastatic small cell carcinoma of the bladderBMC Urology, 2009
- Fibroblast Growth Factor Receptor 1 Promotes Proliferation and Survival via Activation of the Mitogen-Activated Protein Kinase Pathway in Bladder CancerCancer Research, 2009
- Akt Regulates Drug-Induced Cell Death through Bcl-w DownregulationPLOS ONE, 2008
- Oncomirs — microRNAs with a role in cancerNature Reviews Cancer, 2006