Genetic characterization of enterovirus 71 isolated from patients with severe disease by comparative analysis of complete genomes
- 12 April 2012
- journal article
- research article
- Published by Wiley in Journal of Medical Virology
- Vol. 84 (6), 931-939
- https://doi.org/10.1002/jmv.23287
Abstract
Enterovirus 71 (EV71) which causes mild illness in children is also associated with severe neurological complications. This study analyzed the complete genomes of EV71 strains derived from mild and severe diseases in order to determine whether the differences of EV71 genomes were responsible for different clinical presentations. Compared to complete genomes of EV71 strains derived from mild cases (less virulent strains), nucleotide differences in EV71 strains isolated from severe cases (more virulent strains) were observed primarily in the internal ribosomal entry site (IRES) of the 5′‐untranslated region (UTR), which is vital for the cap‐independent translation of viral proteins. In the protein‐coding region, an E–Q substitution at amino acid position 145 of structural protein VP1 that occurred in more than one of more virulent strains was observed. This site is known to be related functionally to receptor binding and virulence in mice. Overall, strains (Group III) isolated from patients with fatal or severe sequelae outcomes had greater sequence substitutions in the 5′‐UTR and/or protein‐coding region and exhibited a relatively low‐average homology to less virulent strains across the entire genome, indicating the possibility of significant genomic diversity in the most virulent EV71 strains. Further studies of EV71 pathogenesis should examine the significance of genomic diversity and the effects of multiple mutations in a viral population. J. Med. Virol. 84:931–939, 2012.Keywords
This publication has 59 references indexed in Scilit:
- Host Factors in Enterovirus 71 ReplicationJournal of Virology, 2011
- The 3C Protein of Enterovirus 71 Inhibits Retinoid Acid-Inducible Gene I-Mediated Interferon Regulatory Factor 3 Activation and Type I Interferon ResponsesJournal of Virology, 2010
- Enterovirus type 71 2A protease functions as a transcriptional activator in yeastJournal of Biomedical Science, 2010
- Evolutionary Genetics of Human Enterovirus 71: Origin, Population Dynamics, Natural Selection, and Seasonal Periodicity of the VP1 GeneJournal of Virology, 2010
- Viral and host proteins involved in picornavirus life cycleJournal of Biomedical Science, 2009
- Reemergence of Enterovirus 71 in 2008 in Taiwan: Dynamics of Genetic and Antigenic Evolution from 1998 to 2008Journal of Clinical Microbiology, 2009
- hnRNP A1 Interacts with the 5′ Untranslated Regions of Enterovirus 71 and Sindbis Virus RNA and Is Required for Viral ReplicationJournal of Virology, 2009
- Far upstream element binding protein 2 interacts with enterovirus 71 internal ribosomal entry site and negatively regulates viral translationNucleic Acids Research, 2008
- Functional Analysis of Picornavirus 2B Proteins: Effects on Calcium Homeostasis and Intracellular Protein TraffickingJournal of Virology, 2008
- Cooperative Effect of the Attenuation Determinants Derived from Poliovirus Sabin 1 Strain Is Essential for Attenuation of Enterovirus 71 in the NOD/SCID Mouse Infection ModelJournal of Virology, 2008