Aptamer–field-effect transistors overcome Debye length limitations for small-molecule sensing
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- 19 October 2018
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 362 (6412), 319-324
- https://doi.org/10.1126/science.aao6750
Abstract
Detection of analytes by means of field-effect transistors bearing ligand-specific receptors is fundamentally limited by the shielding created by the electrical double layer (the “Debye length” limitation). We detected small molecules under physiological high–ionic strength conditions by modifying printed ultrathin metal-oxide field-effect transistor arrays with deoxyribonucleotide aptamers selected to bind their targets adaptively. Target-induced conformational changes of negatively charged aptamer phosphodiester backbones in close proximity to semiconductor channels gated conductance in physiological buffers, resulting in highly sensitive detection. Sensing of charged and electroneutral targets (serotonin, dopamine, glucose, and sphingosine-1-phosphate) was enabled by specifically isolated aptameric stem-loop receptors.Keywords
Funding Information
- National Science Foundation (1509794)
- National Science Foundation (CMMI-1636136)
- National Science Foundation (CBET1026592)
- National Science Foundation (CCF1518715)
- National Institutes of Health (GM104960)
- National Institutes of Health (DA045550)
- CAL-BRAIN (unnumbered)
- National Cancer Institute (CA199849)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK109690)
- Hewlett Packard (unnumbered)
- NantWorks (unnumbered)
- Merkin Family Foundation (unnumbered)
- China Scholarship Council (unnumbered)
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