Engineering eukaryotic protein factories
Open Access
- 21 September 2007
- journal article
- review article
- Published by Springer Science and Business Media LLC in Biotechnology Letters
- Vol. 30 (2), 187-196
- https://doi.org/10.1007/s10529-007-9524-1
Abstract
The biopharmaceuticals market is currently outperforming the pharmaceuticals market and is now valued at US$ 48 billion with an average annual growth of 19%. Behind this success is a 100-fold increase in productivities of eukaryotic expression systems. However, the productivity per cell has remained unchanged for more than 10 years. The engineering of the ER-resident protein folding machinery is discussed together with an overview of signal transduction pathways activated by heterologous protein overexpression to increase cell specific productivities.Keywords
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