Two DNA nanomachines map pH changes along intersecting endocytic pathways inside the same cell
- 26 May 2013
- journal article
- Published by Springer Science and Business Media LLC in Nature Nanotechnology
- Vol. 8 (6), 459-467
- https://doi.org/10.1038/nnano.2013.92
Abstract
International audienceDNA is a versatile scaffold for molecular sensing in living cells, and various cellular applications of DNA nanodevices have been demonstrated. However, the simultaneous use of different DNA nanodevices within the same living cell remains a challenge. Here, we show that two distinct DNA nanomachines can be used simultaneously to map pH gradients along two different but intersecting cellular entry pathways. The two nanomachines, which are molecularly programmed to enter cells via different pathways, can map pH changes within well-defined subcellular environments along both pathways inside the same cell. We applied these nanomachines to probe the pH of early endosomes and the trans-Golgi network, in real time. When delivered either sequentially or simultaneously, both nanomachines localized into and independently captured the pH of the organelles for which they were designed. The successful functioning of DNA nanodevices within living systems has important implications for sensing and therapies in a diverse range of contextsKeywords
This publication has 28 references indexed in Scilit:
- Rab9-dependent retrograde transport and endosomal sorting of the endopeptidase furinJournal of Cell Science, 2011
- A synthetic icosahedral DNA-based host–cargo complex for functional in vivo imagingNature Communications, 2011
- Fission of Tubular Endosomes Triggers Endosomal Acidification and MovementPLOS ONE, 2011
- The Arp2/3 Activator WASH Controls the Fission of Endosomes through a Large Multiprotein ComplexDevelopmental Cell, 2009
- DNA nanomachinesNature Nanotechnology, 2007
- Dynasore, a Cell-Permeable Inhibitor of DynaminDevelopmental Cell, 2006
- Visualization of TGN to Endosome Trafficking through Fluorescently Labeled MPR and AP-1 in Living CellsMolecular Biology of the Cell, 2003
- The End2 mutation in CHO cells slows the exit of transferrin receptors from the recycling compartment but bulk membrane recycling is unaffectedThe Journal of cell biology, 1993
- Brefeldin A induces a microtubule‐dependent fusion of galactosyltransferase‐containing vesicles with the rough endoplasmic reticulumBiology of the Cell, 1991
- Phage antibodies: filamentous phage displaying antibody variable domainsNature, 1990