Quantitative comparison of novel GCaMP-type genetically encoded Ca2+ indicators in mammalian neurons
Open Access
- 1 January 2012
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Cellular Neuroscience
- Vol. 6, 41
- https://doi.org/10.3389/fncel.2012.00041
Abstract
New variants of GCaMP-type genetically encoded Ca2+ indicators (GECIs) have been continuously developed and heavily used in many areas of biology including neuroscience. The latest subfamily called “GECOs” were developed with in vitro high-throughput screening, and shown to have novel spectral properties and/or improved fluorescent responses over their ancestor GCaMP3. The most critical parameter in evaluating performance in neurons, however, remains uncharacterized: the relationship between the GECI responses and the number of action potentials (APs). Here we analyzed the GECI responses to APs in cortical pyramidal cells of mouse acute brain slices. Unexpectedly, we found that none of the GECOs exhibited any improved performance over GCaMP3. Our results imply that careful validation is required for the accurate prediction of the actual performance of GECIs in mammalian neurons. We propose that appropriate guidelines for evaluating their efficacy should be established for the benefit of research community, given the rapidly expanding use of GECIs in neuroscience.Keywords
This publication has 39 references indexed in Scilit:
- Sensorimotor Mismatch Signals in Primary Visual Cortex of the Behaving MouseNeuron, 2012
- Imaging input and output of neocortical networks in vivoProceedings of the National Academy of Sciences of the United States of America, 2005
- Activation of cerebellar parallel fibers monitored in transgenic mice expressing a fluorescent Ca2+ indicator proteinEuropean Journal of Neuroscience, 2005
- Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent proteinNature Biotechnology, 2004
- Monitoring Neural Activity and [Ca2+] with Genetically Encoded Ca2+IndicatorsJournal of Neuroscience, 2004
- Faculty Opinions recommendation of Supralinear Ca2+ influx into dendritic tufts of layer 2/3 neocortical pyramidal neurons in vitro and in vivo.Published by H1 Connect ,2004
- Imaging Calcium Concentration Dynamics in Small Neuronal CompartmentsScience's STKE, 2004
- In vivo two-photon calcium imaging of neuronal networksProceedings of the National Academy of Sciences of the United States of America, 2003
- A high signal-to-noise Ca2+ probe composed of a single green fluorescent proteinNature Biotechnology, 2001
- Spatial profile of dendritic calcium transients evoked by action potentials in rat neocortical pyramidal neurones.The Journal of Physiology, 1995