Study of novel chitosan‐gelatin artificial skin in vitro
- 20 December 2002
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research Part A
- Vol. 64A (2), 301-308
- https://doi.org/10.1002/jbm.a.10223
Abstract
A novel absorbable scaffold composed of chitosan and gelatin was fabricated by freezing and lyophilizing methods, resulting in an asymmetric structure. This bilaminar texture is suitable for preparing a bilayer skin substitute. The methods employed to confirm the applicability of this chitosan-gelatin scaffold as an ideal skin substitute were a water uptake ability test, in vitro fibroblast proliferation, and scaffold tests in which fibroblasts were co-cultured with keratinocytes. The chitosan-gelatin scaffolds were more wettable and adsorbed more water than did chitosan alone. In static cell culture the thinner scaffold is better than the thicker one, and because of diffusion limitations in the scaffold, culture time must be within 3 weeks before transplantation to living tissues. Keratinocytes were co-cultured with fibroblasts in chitosan-gelatin scaffolds to construct an artificial bilayer skin in vitro. The artificial skin obtained was flexible and had good mechanical properties. Moreover, there was no contraction observed in the in vitro cell culture tests. The data from this study suggest that chitosan-gelatin scaffolds are suitable for skin tissue engineering goals. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 64A: 301–308, 2003This publication has 16 references indexed in Scilit:
- Skin substitutes: a reviewBurns, 2001
- Collagen based dressings — a reviewBurns, 2000
- A new model for studying differentiation and growth of epidermal cultures on hyaluronan-based carrierBiomaterials, 1999
- Skin: The First Tissue-Engineered ProductsScientific American, 1999
- Fibroblast seeding and culture in biodegradable porous substratesJournal of Applied Biomaterials, 1995
- Optimization of thickness, pore size and mechanical properties of a biomaterial designed for deep burn coverageClinical Materials, 1994
- Biochemical significance of exogenous chitins and chitosans in animals and patientsCarbohydrate Polymers, 1993
- Lysozyme degradation of partially deacetylated chitin, its films and hydrogelsBiomaterials, 1982
- Collagen sponge: Theory and practice of medical applicationsJournal of Biomedical Materials Research, 1977
- Seria cultivation of strains of human epidemal keratinocytes: the formation keratinizin colonies from single cell isCell, 1975