Comparative Absorption of 2-Hydroxy-4-(Methylthio)-butanoic Acid and L-Methionine in the Broiler Chick
- 1 November 1984
- journal article
- research article
- Published by Elsevier BV in Journal of Nutrition
- Vol. 114 (11), 2179-2186
- https://doi.org/10.1093/jn/114.11.2179
Abstract
The absorption of [1-14C]2-hydroxy-4-(methylthio)butanoic acid (HMB) and L-methionine (L-Met) was evaluated in the broiler chick. Methods included in vitro intestinal tissue uptake and appearance in the plasma from ligated intestinal segments in situ, and from crop intubations. Intestinal uptake of L-Met was partially inhibited with 2,4-dinitrophenol, while HMB uptake was unaffected. The uptake of L-Met conformed to Michaelis-Menten kinetics except that no plateau was reached. This indicated that L-Met absorption may be accomplished by both concentration and energy-dependent processes. The in vitro uptake of HMB in relation to concentration was linear indicating that its absorption was concentration dependent. Absorption of HMB and L-Met after crop intubation indicated higher plasma concentrations of HMB than of L-Met, particularly at higher doses. Both compounds were absorbed at similar rates at concentrations 50–100 times below physiological concentrations and absorption rates were different at different small intestinal locations. These results indicate that the ability to use HMB as a source of methionine would not be limited by absorption.Keywords
This publication has 13 references indexed in Scilit:
- Relative Biopotency of Three Methionine Sources for Laying HensPoultry Science, 1982
- Effectiveness of the Free Acid of Methionine Hydroxy Analogue as a Methionine Supplement in Broiler DietsPoultry Science, 1981
- Specificity relationships in cystine transport in the chicken intestineComparative Biochemistry and Physiology Part A: Physiology, 1978
- Saturation kinetics and competition of amino acid uptake in the domestic fowl (Gallus domesticus)Comparative Biochemistry and Physiology Part A: Physiology, 1977
- Intestinal absorption of glutamic acid in the chickenComparative Biochemistry and Physiology Part A: Physiology, 1977
- Sulfur Amino Acid Activity of Glutathione, DL- -Hydroxy-Methionine, and -Keto-Methionine in ChicksExperimental Biology and Medicine, 1977
- A comparison of intestinal amino acid absorption in various avian and mammalian speciesComparative Biochemistry and Physiology Part A: Physiology, 1976
- The intestinal absorption of methionine in chickens provided with permanent Thiry-Vella fistulasCellular and Molecular Life Sciences, 1969
- Methionine Hydroxy Analogue and l-Methiomne in Broiler DietsPoultry Science, 1968
- The uptake of amino acids by isolated segments of rat intestine I. A survey of factors affecting the measurement of uptakeBiochimica et Biophysica Acta, 1960