Purification, characterization and procoagulant activity of polysaccharides from Angelica dahurice roots
Open Access
- 10 February 2017
- journal article
- research article
- Published by Springer Science and Business Media LLC in Chemistry Central Journal
- Vol. 11 (1), 1-8
- https://doi.org/10.1186/s13065-017-0243-y
Abstract
Five polysaccharides, namely ADPs-1a, ADPs-1b, ADPs-2, ADPs-3a and ADPs-3b, were extracted from Angelicae dahuricae Radix, purified, and identified by high performance gel permeation chromatography (HPSEC), gas chromatography (GC), Fourier transform infrared (FT-IR) spectrometer and nuclear magnetic resonance spectra (NMR), including the determination of procoagulant activity in vitro. The average molecular weight (Mw) of the polysaccharides was 153,800, 8312, 111,700, 3766 and 96,680 g/mol, respectively. Coagulation assays indicated that ADPs-1b, ADPs-2, ADPs-3a and ADPs-3b had procoagulant activities. ADPs-1b exerted the procoagulant activities through intrinsic pathway, extrinsic pathway and increased the content of FIB in vitro. ADPs-2 exerted the procoagulant activities through intrinsic pathway and extrinsic pathway. ADPs-3a had procoagulant activities and the activity was associated with the intrinsic pathway and increased the content of FIB. ADPs-3b exerted the activities through extrinsic pathway and increased the content of FIB.Keywords
Funding Information
- Henan Province University Science and Technology Innovation Team (16IRTSTHN019)
- Key project in Science and Technology Agency of Kaifeng City (1608003)
- Kaifeng City Science and Technology Innovation Talent (1509010)
- National cooperation project of Henan province (2015GH12)
- Key Project in Science and Technology Agency of Henan Province (172102310610)
This publication has 2 references indexed in Scilit: