An early intermediate of refolding α‐lactalbumin forms within 20 ms
- 2 November 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 223 (2), 327-329
- https://doi.org/10.1016/0014-5793(87)80313-7
Abstract
The kinetics of α-lactalbumin refolding were studied by the stopped-flow method with the registration of CD and intrinsic fluorescence at several wavelengths. It was shown that the early kinetic intermediate forms during the dead-time of the experiment (20 ms). This intermediate has a considerable amount of secondary structure and unpolar clusters in its molecular structure but has no rigid tertiary structure.Keywords
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