High-density lipoprotein cholesterol assay by magnesium dextransulphate precipitation
- 1 January 1986
- journal article
- research article
- Published by Informa UK Limited in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 46 (8), 715-721
- https://doi.org/10.3109/00365518609084042
Abstract
A method for determination of high-density lipoprotein cholesterol (HDL-C) is evaluated: after precipitation of low and very low lipoprotein cholesterol in serum by magnesium dextransulphate, HDL-C is determined by an enzymatic method. The precision of the method was good: coefficient of variation 4%. The accuracy was good, evaluated by correlating HDL-C results from the present assay to the results obtained from three other HDL-C assays (n=17): ultracentrifugation r=0.91, phosphotungstate/MgCl2 r=0.98, and α-lipoprotein determination by electrophoresis r=0.91 (p<0.01). Repeated analysis showed that serum may be kept at 4 °C for 1 month, at -20 °C for 2 months, and requires -80 °C for longer storage. Ten patients with acute myocardial infarction showed significantly lower HDL-C from day 4 in the acute phase and during the first 3 months follow up (p<0·05). Eighty patients with peripheral vascular disease, who were compared to a group of matched controls, also showed significantly decreased serum HDL-C (p<0·01). The present HDL-C assay is easy, fast and reliable and is considered a valuable clinical test.Keywords
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