Sp1‐dependent regulation of Myeloid Elf‐1 like factor in human epithelial cells
- 21 April 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (13), 2811-2816
- https://doi.org/10.1016/j.febslet.2005.04.015
Abstract
Myeloid Elf-1 like factor (MEF) is an ETS protein, which activates the promoters of granulocyte macrophage colony-stimulating factor, interleukin-3, lysozyme, human beta defensin-2 and perforin. In spite of its many known functions, little is known about MEF transcriptional regulation. Here, we cloned the 5'-flanking region of human MEF gene and identified a TATA-less promoter region at -204/-54 which contains 4 putative binding sites for Sp1, two of which are essential in up-regulating MEF activity. These were proven by EMSA and blocking Sp1 using RNAi or mithramycin A treatment of HEK293 cells. Our results suggest that Sp1 constitutively regulates the MEF gene.Keywords
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