Biophysical properties of mitochondrial fusion events in pancreatic β-cells and cardiac cells unravel potential control mechanisms of its selectivity
- 1 August 2010
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Cell Physiology
- Vol. 299 (2), C477-C487
- https://doi.org/10.1152/ajpcell.00427.2009
Abstract
Studies in various types of cells find that, on average, each mitochondrion becomes involved in a fusion event every 15 min, depending on the cell type. As most contact events do not result in mitochondrial fusion, it is expected that properties of the individual mitochondrion determine the likelihood of a fusion event. However, apart from membrane potential, the properties that influence the likelihood of entering a fusion event are not known. Here, we tag and track individual mitochondria in H9c2, INS1, and primary β-cells and determine the biophysical properties that increase the likelihood of a fusion event. We found that the probability for fusion is independent of contact duration and organelle dimensions, but it is influenced by organelle motility. Furthermore, the history of a previous fusion event of the individual mitochondrion influenced both the likelihood for a subsequent fusion event, as well as the site on the mitochondrion at which the fusion occurred. These observations unravel the specific properties that distinguish mitochondria that will enter fusion events from the ones that will not. Altogether, these properties may help to elucidate the molecular mechanisms that regulate fusion at the level of the single mitochondrion.Keywords
This publication has 55 references indexed in Scilit:
- Mitochondrial ‘kiss-and-run’: interplay between mitochondrial motility and fusion–fission dynamicsThe EMBO Journal, 2009
- Frequency and Selectivity of Mitochondrial Fusion Are Key to Its Quality Maintenance FunctionBiophysical Journal, 2009
- SLP-2 is required for stress-induced mitochondrial hyperfusionThe EMBO Journal, 2009
- Bcl-xL increases mitochondrial fission, fusion, and biomass in neuronsThe Journal of cell biology, 2009
- Quality control of mitochondria: protection against neurodegeneration and ageingThe EMBO Journal, 2008
- Fission and selective fusion govern mitochondrial segregation and elimination by autophagyThe EMBO Journal, 2008
- Regulation of the mitochondrial dynamin-like protein Opa1 by proteolytic cleavageThe Journal of cell biology, 2007
- OPA1 processing controls mitochondrial fusion and is regulated by mRNA splicing, membrane potential, and Yme1LThe Journal of cell biology, 2007
- Regulation of mitochondrial morphology through proteolytic cleavage of OPA1The EMBO Journal, 2006
- Composition and Dynamics of Human Mitochondrial NucleoidsMolecular Biology of the Cell, 2003