PHOTOPHYSICAL STUDIES ON THE BINDING OF TETRASULFONATOPHENYLPORPHYRIN TO LENS PROTEINS
- 1 October 1990
- journal article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 52 (4), 845-848
- https://doi.org/10.1111/j.1751-1097.1990.tb08691.x
Abstract
Previous studies have shown that mesotetra(p-sulfonatophenyl)porphine (TPPS) binds to lens proteins. This characteristic should increase the residence time of the sensitizer in the lens and therefore enhance the probability of inducing photooxidative damage to that tissue in vivo. Subsequent in vivo studies have verified that contention. The present studies were performed to determine the effect of such binding on the spectroscopy and photophysics of the porphyrins. It was found that the binding of TPPS (1) quenches the fluorescence of lens proteins, (2) causes a shift in the ground state absorption spectra, fluorescence excitation spectra and the triplet excited state spectrum of TPPS to longer wavelengths and (3) results in an increase in the triplet state lifetime of TPPS. In the presence of the isolated crystallins the average triplet lifetime increases in the following order: gamma less than beta less than alpha.Keywords
This publication has 9 references indexed in Scilit:
- PHOTOPHYSICAL PROPERTIES OF meso‐TETRAPHENYLPORPHYRIN and SOME meso‐TETRA(HYDROXYPHENYL)PORPHYRINSPhotochemistry and Photobiology, 1988
- IN VITRO STUDIES ON THE PHOTOSENSITIZED OXIDATION OF LENS PROTEINS BY PORPHYRINSPhotochemistry and Photobiology, 1987
- Laser flash photolysis of DNA-intercalated ethidium bromide in the presence of methylviologenThe Journal of Physical Chemistry, 1987
- TUMOR LOCALIZATION AND PHOTOSENSITIZATION BY SULFONATED DERIVATIVES OF TETRAPHENYLPORPHINEPhotochemistry and Photobiology, 1987
- The Photochemistry of Haematoporphyrin and Related SystemsInternational Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1986
- Photophysics and Photochemistry of Hematoporphyrin, Hematoporphyrin Derivative and Uroporphyrin IAdvances in experimental medicine and biology, 1985
- Quantatiye Studies of Tetraphenytlporphinesulfonate and Hematoporphyrin Derivative Distribution in Animal Tumor SystemsAdvances in experimental medicine and biology, 1985
- FLASH PHOTOLYSIS STUDIES OF HEMATO‐AND COPRO‐PORPHYRINS IN HOMOGENEOUS AND MICROHETEROGENEOUS AQUEOUS DISPERSIONSPhotochemistry and Photobiology, 1983
- Human α-crystallin. II. Characterization of the protein isolated from the periphery of cataractous lensesBiochemistry, 1976