Tissue factor-positive neutrophils bind to injured endothelial wall and initiate thrombus formation
Top Cited Papers
Open Access
- 5 September 2012
- journal article
- research article
- Published by American Society of Hematology in Blood
- Vol. 120 (10), 2133-2143
- https://doi.org/10.1182/blood-2012-06-437772
Abstract
For a long time, blood coagulation and innate immunity have been viewed as interrelated responses. Recently, the presence of leukocytes at the sites of vessel injury has been described. Here we analyzed interaction of neutrophils, monocytes, and platelets in thrombus formation after a laser-induced injury in vivo. Neutrophils immediately adhered to injured vessels, preceding platelets, by binding to the activated endothelium via leukocyte function antigen-1-ICAM-1 interactions. Monocytes rolled on a thrombus 3 to 5 minutes postinjury. The kinetics of thrombus formation and fibrin generation were drastically reduced in low tissue factor (TF) mice whereas the absence of factor XII had no effect. In vitro, TF was detected in neutrophils. In vivo, the inhibition of neutrophil binding to the vessel wall reduced the presence of TF and diminished the generation of fibrin and platelet accumulation. Injection of wild-type neutrophils into low TF mice partially restored the activation of the blood coagulation cascade and accumulation of platelets. Our results show that the interaction of neutrophils with endothelial cells is a critical step preceding platelet accumulation for initiating arterial thrombosis in injured vessels. Targeting neutrophils interacting with endothelial cells may constitute an efficient strategy to reduce thrombosis. (Blood. 2012; 120(10):2133-2143)Keywords
This publication has 46 references indexed in Scilit:
- ‘Slings’ enable neutrophil rolling at high shearNature, 2012
- Monocytes, neutrophils, and platelets cooperate to initiate and propagate venous thrombosis in mice in vivoThe Journal of Experimental Medicine, 2012
- Neutrophil extracellular traps promote deep vein thrombosis in miceJournal of Thrombosis and Haemostasis, 2011
- Increased Efficacy of Breast Cancer Chemotherapy in Thrombocytopenic MiceCancer Research, 2011
- Extracellular DNA traps promote thrombosisProceedings of the National Academy of Sciences, 2010
- Platelet Polyphosphates Are Proinflammatory and Procoagulant Mediators In VivoCell, 2009
- Cancer cell–derived microparticles bearing P-selectin glycoprotein ligand 1 accelerate thrombus formation in vivoThe Journal of Experimental Medicine, 2009
- Tissue factor-dependent coagulation contributes to α-naphthylisothiocyanate-induced cholestatic liver injury in miceAmerican Journal of Physiology-Gastrointestinal and Liver Physiology, 2009
- Trans-cellular migration: cell–cell contacts get intimateCurrent Opinion in Cell Biology, 2008
- Par4 is required for platelet thrombus propagation but not fibrin generation in a mouse model of thrombosisProceedings of the National Academy of Sciences, 2007