DNA sequence for a low-level promoter of the lac repressor gene and an ‘up’ promoter mutation
- 1 August 1978
- journal article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 274 (5673), 762-765
- https://doi.org/10.1038/274762a0
Abstract
The promoter for a weakly expressed constitutive gene, the lactose repressor gene (lacI), has been sequenced, along with an 'up' promoter mutation Iq. The 10-fold enhancement in I expression found in Iq is the result of a single base change at position -35. To facilitate the sequencing, the lacI gene was cloned in a small plasmid.Keywords
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