Controlling Cellular Uptake by Surface Chemistry, Size, and Surface Topology at the Nanoscale
- 28 October 2009
- Vol. 5 (21), 2424-2432
- https://doi.org/10.1002/smll.200900578
Abstract
Cell cytosol and the different subcellular organelles house the most important biochemical processes that control cell functions. Effective delivery of bioactive agents within cells is expected to have an enormous impact on both gene therapy and the future development of new therapeutic and/or diagnostic strategies based on single‐cell–bioactive‐agent interactions. Herein a biomimetic nanovector is reported that is able to enter cells, escape from the complex endocytic pathway, and efficiently deliver actives within clinically relevant cells without perturbing their metabolic activity. This nanovector is based on the pH‐controlled self‐assembly of amphiphilic copolymers into nanometer‐sized vesicles (or polymersomes). The cellular‐uptake kinetics can be regulated by controlling the surface chemistry, the polymersome size, and the polymersome surface topology. The latter is controlled by the extent of polymer–polymer phase separation within the external envelope of the polymersome.This publication has 32 references indexed in Scilit:
- Size‐Dependent Endocytosis of NanoparticlesAdvanced Materials, 2009
- Kinetics of pH-Induced Formation and Dissociation of Polymeric Vesicles Assembled from a Water-Soluble Zwitterionic Diblock CopolymerLangmuir, 2008
- Cholesterol transport from liposomal delivery vehiclesBiomaterials, 2007
- Cellular uptake of functionalized carbon nanotubes is independent of functional group and cell typeNature Nanotechnology, 2007
- A Guide to Accurate Fluorescence Microscopy Colocalization MeasurementsBiophysical Journal, 2006
- Design and development of polymers for gene deliveryNature Reviews Drug Discovery, 2005
- Synthesis of controlled-structure sulfate-based copolymers via atom transfer radical polymerisation and their use as crystal habit modifiers for BaSO4Journal of Materials Chemistry, 2002
- Lyotropic Phase Morphologies of Amphiphilic Block CopolymersMacromolecules, 2001
- The requirement for basement membrane antigens in the production of human epidermal/dermal composites in vitroBritish Journal of Dermatology, 1999
- Cellular uptake of the tat protein from human immunodeficiency virusCell, 1988