Analysis of DNA Content and BrdU Incorporation
Open Access
- 1 October 1997
- journal article
- unit
- Published by Wiley in Current Protocols in Cytometry
- Vol. 2 (1), 7.7.1-7.7.9
- https://doi.org/10.1002/0471142956.cy0707s02
Abstract
During the past two decades, the techniques of analysis of cell cycle kinetics based upon the flow cytometric detection of BrdU incorporation have replaced the cumbersome autoradiographic methodology utilizing tritiated thymidine. This unit distills the knowledge gained over this time into concise and easy to follow protocols for DNA content analysis following thermal denaturation or acid denaturation, as well as a modified method that combines thermal denaturation with washless immunocytochemical detection of BrdU.Keywords
This publication has 14 references indexed in Scilit:
- Detection of Apoptosis and DNA Replication by Differential Labeling of DNA Strand Breaks with Fluorochromes of Different ColorExperimental Cell Research, 1996
- Labelling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferationCell Proliferation, 1995
- Chapter 19 Immunochemical Quantitation of Bromodeoxyuridine: Application to Cell–Cycle KineticsPublished by Elsevier BV ,1994
- Washless double staining of unfixed nuclei for flow cytometric analysis of DNA and a nuclear antigen (Ki‐67 or bromodeoxyuridine)Cytometry, 1991
- Use of restriction endonucleases and exonuclease III to expose halogenated pyrimidines for immunochemical stainingCytometry, 1988
- An improved immunocytochemical procedure for high‐sensitivity detection of incorporated bromodeoxyuridineCytometry, 1987
- Detection of 5-bromodeoxyuridine (BrdUrd) incorporation by monoclonal antibodies: role of the DNA denaturation step.Journal of Histochemistry & Cytochemistry, 1985
- Monoclonal Antibody to 5-Bromo- and 5-Iododeoxyuridine: A New Reagent for Detection of DNA ReplicationScience, 1982
- Discrimination of cycling and non-cycling lymphocytes by BUdR-suppressed acridine orange fluorescence in a flow cytometric systemExperimental Cell Research, 1978
- Fluorometric detection of deoxyribonucleic acid synthesis; possibilities for interfacing bromodeoxyuridine dye techniques with flow fluorometry.Journal of Histochemistry & Cytochemistry, 1977