1H NMR spectroscopy in the diagnosis of Pseudomonas aeruginosa‐induced urinary tract infection
- 22 July 2005
- journal article
- clinical trial
- Published by Wiley in NMR in Biomedicine
- Vol. 18 (5), 293-299
- https://doi.org/10.1002/nbm.957
Abstract
The utility of 1H NMR spectroscopy is suggested and demonstrated for the diagnosis of Pseudomonas aeruginosa in urinary tract infection (UTI). The specific property of P. aeruginosa of metabolizing nicotinic acid to 6‐hydroxynicotinic acid (6‐OHNA) is exploited. The quantity of 6‐OHNA produced correlates well with the viable bacterial count. Other common bacteria causing UTI such as Escherichia coli, Klebsiella pneumonia, Enterobacter aerogenes, Acinetobacter baumanii, Proteus mirabilis, Citrobacter frundii, Enterococcus faecalis, Streptococcus gp B and Staphylococcus aureus do not metabolize nicotinic acid under similar conditions. The method provides a single‐step documentation of P. aeruginosa qualitatively as well as quantitatively. The NMR method is demonstrated on urine samples from 30 patients with UTI caused by P. aeruginosa. Copyright © 2005 John Wiley & Sons, Ltd.Keywords
This publication has 17 references indexed in Scilit:
- Metabonomics and its role in drug development and disease diagnosisExpert Review of Molecular Diagnostics, 2004
- Field evaluation of a second-generation cytometer UF-100 in diagnosis of acute urinary tract infections in adult patientsClinical Microbiology & Infection, 2002
- National Nosocomial Infections Surveillance (NNIS) Report, data summary from October 1986–April 1996, issued May 1996: A report from the National Nosocomial Infections Surveillance (NNIS) SystemAmerican Journal of Infection Control, 1996
- Crisis in Infectious Diseases: Time for a New Paradigm?Clinical Infectious Diseases, 1996
- Pyrolysis mass spectrometry and its applications in biotechnologyCurrent Opinion in Biotechnology, 1996
- UV Resonance Raman Studies of BacteriaApplied Spectroscopy Reviews, 1992
- Analyses of the FlashTrack DNA probe and UTIscreen bioluminescence tests for bacteriuriaJournal of Clinical Microbiology, 1992
- Laboratory in the Diagnosis and Management of Urinary Tract InfectionsMedical Clinics of North America, 1991
- Classification and identification of bacteria by Fourier-transform infrared spectroscopyMicrobiology, 1991
- Availability of 6-hydroxynicotinic acid for rapid identification of pseudomonas aeruginosa and serratia marcescensJournal of Chromatography B: Biomedical Sciences and Applications, 1985