Isolation, Purification, and Characterization of Xylanase Produced by a New Species ofBacillusin Solid State Fermentation
Open Access
- 1 January 2012
- journal article
- research article
- Published by Hindawi Limited in International Journal of Microbiology
- Vol. 2012, 1-8
- https://doi.org/10.1155/2012/683193
Abstract
A thermoalkalophilic new species ofBacillus, similar toBacillus arseniciselenatisDSM 15340, produced extracellular xylanase under solid state fermentation when wheat bran is used as carbon source. The extracellular xylanase was isolated by ammonium sulfate (80%) precipitation and purified using ion exchange chromatography. The molecular weight of xylanase was ~29.8 ;kDa. The optimum temperature and pH for the enzyme activity were 50°C and pH 8.0. The enzyme was active on birchwood xylan and little active onp-nitrophenyl xylopyranoside but not on Avicel, CMC, cellobiose, and starch, showing its absolute substrate specificity. For birchwood xylan, the enzyme gave a Km 5.26 ;mg/mL and Vmax 277.7 ;μmol/min/mg, respectively. In addition, the xylanase was also capable of producing high-quality xylo-oligosaccharides, which indicated its application potential not only in pulp biobleaching processes but also in the nutraceutical industry.Keywords
Funding Information
- Tatyasaheb Kore Institute of Engineering and Technology, India
This publication has 43 references indexed in Scilit:
- Purification and characterization of extracellular xylanase from Streptomyces cyaneus SN32Bioresource Technology, 2008
- Production and partial characterization of cellulase free xylanase by Bacillus subtilis C 01 using agriresidues and its application in biobleaching of nonwoody plant pulpsLetters in Applied Microbiology, 2007
- Purification of xylanase from alkaliphilic Bacillus sp. K-8 by using corn husk columnProcess Biochemistry, 2006
- Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substratesJournal of Microbiological Methods, 2004
- Production of Aspergillus xylanase by lignocellulosic waste fermentation and its applicationJournal of Applied Microbiology, 1999
- Molecular and biotechnological aspects of xylanasesFEMS Microbiology Reviews, 1999
- Measurement of cellulase activitiesPure and Applied Chemistry, 1987
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Use of Dinitrosalicylic Acid Reagent for Determination of Reducing SugarAnalytical Chemistry, 1959
- The Determination of Enzyme Dissociation ConstantsJournal of the American Chemical Society, 1934