Innovations in the Imaging of Brain Functions using Fluorescent Proteins
- 20 October 2005
- journal article
- review article
- Published by Elsevier BV in Neuron
- Vol. 48 (2), 189-199
- https://doi.org/10.1016/j.neuron.2005.10.003
Abstract
Fluorescence imaging has enabled us to decipher spatiotemporal information coded in complex tissues. Genetically encoded probes that enable fluorescence imaging of excitable cell activity have been constructed by fusing fluorescent proteins to functional proteins that are involved in physiological signaling. The probes are introduced into an intact organism and targeted to specific tissues, cell types, or subcellular compartments, thereby allowing specific signals to be extracted more efficiently than was previously possible. In this primer, I will describe how this approach has met neuroscientists' demands and desiresKeywords
This publication has 100 references indexed in Scilit:
- Regulated Fast Nucleocytoplasmic Shuttling Observed by Reversible Protein HighlightingScience, 2004
- Tracking SNARE Complex Formation in Live Endocrine CellsScience, 2004
- In Vivo Imaging of Neuronal Activity by Targeted Expression of a Genetically Encoded Probe in the MouseNeuron, 2004
- FRET or No FRET: A Quantitative ComparisonBiophysical Journal, 2003
- An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent proteinProceedings of the National Academy of Sciences of the United States of America, 2002
- A monomeric red fluorescent proteinProceedings of the National Academy of Sciences of the United States of America, 2002
- A Genetically Targetable Fluorescent Probe of Channel Gating with Rapid KineticsBiophysical Journal, 2002
- Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed)Nature Biotechnology, 2002
- Circular permutation and receptor insertion within green fluorescent proteinsProceedings of the National Academy of Sciences of the United States of America, 1999
- Design of Organic Molecules with Large Two-Photon Absorption Cross SectionsScience, 1998