Multichromosomal Genome Structure and Confirmation of Diazotrophy in Novel Plant-Associated Burkholderia Species
- 15 July 2008
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 74 (14), 4574-4579
- https://doi.org/10.1128/aem.00201-08
Abstract
Pulsed-field gel electrophoresis and 16S rRNA hybridization experiments showed that multichromosome genome structures and very large genome sizes (6.46 to 8.73 Mb) are prevalent in novel plant-associated Burkholderia species. 15 N 2 isotope dilution assays revealed unambiguous diazotrophy in these novel species. nifH gene sequence analysis, often used to determine phylogenetic relatedness among diazotrophs, showed tight clusters of Burkholderia species, which were clearly distinct from those of other diazotrophs.Keywords
This publication has 30 references indexed in Scilit:
- The Tomato Rhizosphere, an Environment Rich in Nitrogen-Fixing Burkholderia Species with Capabilities of Interest for Agriculture and BioremediationApplied and Environmental Microbiology, 2007
- Monophyly of nodA and nifH Genes across Texan and Costa Rican Populations of Cupriavidus Nodule SymbiontsApplied and Environmental Microbiology, 2007
- Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatilityProceedings of the National Academy of Sciences of the United States of America, 2006
- Diazotrophic Burkholderia Species Associated with Field-Grown Maize and SugarcaneApplied and Environmental Microbiology, 2006
- Changes in agricultural management drive the diversity of Burkholderia species isolated from soil on PCAT mediumSoil Biology and Biochemistry, 2006
- Vibrios Commonly Possess Two ChromosomesJournal of Bacteriology, 2005
- The multifarious, multireplicon Burkholderia cepacia complexNature Reviews Microbiology, 2005
- Comparative Genomic Structure of ProkaryotesAnnual Review of Genetics, 2004
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975