Bacterial CS2 Hydrolases from Acidithiobacillus thiooxidans Strains Are Homologous to the Archaeal Catenane CS2 Hydrolase
Open Access
- 8 July 2013
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 195 (18), 4046-4056
- https://doi.org/10.1128/jb.00627-13
Abstract
Carbon disulfide (CS2) and carbonyl sulfide (COS) are important in the global sulfur cycle, and CS2 is used as a solvent in the viscose industry. These compounds can be converted by sulfur-oxidizing bacteria, such as Acidithiobacillus thiooxidans species, to carbon dioxide (CO2) and hydrogen sulfide (H2S), a property used in industrial biofiltration of CS2-polluted airstreams. We report on the mechanism of bacterial CS2 conversion in the extremely acidophilic A. thiooxidans strains S1p and G8. The bacterial CS2 hydrolases were highly abundant. They were purified and found to be homologous to the only other described (archaeal) CS2 hydrolase from Acidianus strain A1-3, which forms a catenane of two interlocked rings. The enzymes cluster in a group of β-carbonic anhydrase (β-CA) homologues that may comprise a subclass of CS2 hydrolases within the β-CA family. Unlike CAs, the CS2 hydrolases did not hydrate CO2 but converted CS2 and COS with H2O to H2S and CO2. The CS2 hydrolases of A. thiooxidans strains G8, 2Bp, Sts 4-3, and BBW1, like the CS2 hydrolase of Acidianus strain A1-3, exist as both octamers and hexadecamers in solution. The CS2 hydrolase of A. thiooxidans strain S1p forms only octamers. Structure models of the A. thiooxidans CS2 hydrolases based on the structure of Acidianus strain A1-3 CS2 hydrolase suggest that the A. thiooxidans strain G8 CS2 hydrolase may also form a catenane. In the A. thiooxidans strain S1p enzyme, two insertions (positions 26 and 27 [PD] and positions 56 to 61 [TPAGGG]) and a nine-amino-acid-longer C-terminal tail may prevent catenane formationKeywords
This publication has 65 references indexed in Scilit:
- MOLEonline 2.0: interactive web-based analysis of biomacromolecular channelsNucleic Acids Research, 2012
- Autotrophic Methanotrophy in Verrucomicrobia: Methylacidiphilum fumariolicumSolV Uses the Calvin-Benson-Bassham Cycle for Carbon Dioxide FixationJournal of Bacteriology, 2011
- MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony MethodsMolecular Biology and Evolution, 2011
- Horizontal Transfer, Not Duplication, Drives the Expansion of Protein Families in ProkaryotesPLoS Genetics, 2010
- Carbonic Anhydrase Is Essential forStreptococcus pneumoniaeGrowth in Environmental Ambient AirJournal of Bacteriology, 2010
- A review of health effects of carbon disulfide in viscose industry and a proposal for an occupational exposure limitCritical Reviews in Toxicology, 2009
- Biogeography of theSulfolobus islandicuspan-genomeProceedings of the National Academy of Sciences of the United States of America, 2009
- Discovery of a Thermophilic Protein Complex Stabilized by Topologically Interlinked ChainsJournal of Molecular Biology, 2007
- MUSCLE: multiple sequence alignment with high accuracy and high throughputNucleic Acids Research, 2004
- Robustness of a full‐scale biological system treating industrial CS2 emissionsEnvironmental Progress, 2003