Detection of Wuchereria bancrofti in mosquitoes by the polymerase chain reaction: a potentially useful tool for large-scale control programmes
- 1 January 2001
- journal article
- Published by Oxford University Press (OUP) in Transactions of the Royal Society of Tropical Medicine and Hygiene
- Vol. 95 (1), 29-32
- https://doi.org/10.1016/s0035-9203(01)90322-0
Abstract
Focally endemic bancroftian filariasis is targeted for elimination in the Nile delta of Egypt. Improved methods are needed for identifying endemic villages to be included in the control programme and for monitoring its success. We have evaluated the performance of a polymerase chain reaction (PCR) assay in estimating Wuchereria bancrofti infection in pools of Culex pipiens (1–25 females) from 2 adjacent villages with high (El Qolzom, 10 · 8%) and low (Kafr Shorafa, 2 · 1%) prevalence rates of human filariasis. This assay detects a repeated sequence in W. bancrofti deoxyribonucleic acid (DNA). Mosquitoes resting within houses were captured by aspiration and pooled by house. Houses were classified as positive or negative for human filarial infection based on night blood examinations of residents. The assay detected parasite DNA in mosquitoes from 60% of 25 infected houses and 24% of 25 uninfected houses. PCR processing of mosquitoes caught within houses of unknown filariasis infection status (44 in El Qolzom, 37 in Kafr Shorafa) identified 31 · 8% and 8 · 1% of houses, respectively, as containing infected mosquitoes. These results support the validity of the PCR assay for evaluating filarial prevalence in different villages. C. pipiens collected outdoors in dry ice-baited traps and tested by PCR (266 in Qolzom, 82 in Kafr Shorafa) did not contain parasite DNA. Pools of female mosquitoes (296 in Qolzom, 240 in Kafr Shorafa) captured in oviposition traps were also negative. We concluded that the PCR based assay is a powerful epidemiological tool that can be used for evaluating W. bancrofti infection in villages in the Nile delta and for monitoring the application of control programmes in filariasis endemic areas.Keywords
This publication has 7 references indexed in Scilit:
- A longitudinal study of Bancroftian filariasis in the Nile Delta of Egypt: baseline data and one-year follow-up.The American Journal of Tropical Medicine and Hygiene, 1999
- Randomised community-based trial of annual single-dose diethylcarbamazine with or without ivermectin against Wuchereria bancrofti infection in human beings and mosquitoesThe Lancet, 1998
- A polymerase chain reaction-based assay for detection of Wuchereria bancrofti in human blood and Culex pipiensTransactions of the Royal Society of Tropical Medicine and Hygiene, 1997
- A Polymerase Chain Reaction Assay for Detection of the Parasite Wuchereria bancrofti in Human Blood SamplesThe American Journal of Tropical Medicine and Hygiene, 1996
- Host-Feeding Patterns of Culex pipiens and Cx. antennatus (Diptera: Culicidae) from a Village in Sharqiya Governorate, EgyptJournal of Medical Entomology, 1995
- Detection of Wuchereria bancrofti larvae in pools of mosquitoes by the polymerase chain reactionTransactions of the Royal Society of Tropical Medicine and Hygiene, 1994
- Host-Feeding Patterns of Culex Mosquitoes (Diptera: Culicidae) on Farms in Gharbiya Governorate, Egypt12Journal of Medical Entomology, 1985