An Enzyme Sensor For Determination of Acetylcholine and Choline In Rat Brain Extracts

Abstract
A choline enzyme sensor, recently developed by the authors, was used for choline and acetylcholine determination in rat brain extracts, using choline oxidase immobilized on cellulose triacetate membranes, and acetylcholinesterase in homogeneous solution. the method proved useful for assay of the acetylcholine content in a commercial pharmaceutical formulation used in ophthalmology.