Differential diagnosis of cutaneous carcinomas by infrared spectral micro-imaging combined with pattern recognition
- 24 February 2009
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 134 (6), 1208-1214
- https://doi.org/10.1039/b820998g
Abstract
Non-melanoma skin cancer includes basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and Bowen's disease. The differential diagnosis of these lesions is sometimes difficult and relies on the histopathological examination of surgical specimens. However, a precise differential diagnosis is crucial for an accurate therapy and thus better patient care. FTIR spectral micro-imaging was applied directly on formalin-fixed paraffin-embedded samples of non-melanoma skin cancers. Chemometric and multivariate statistical analyses were developed to generate an automated IR-based histology without any chemical dewaxing. Different prediction models were developed using linear discriminant analysis combined with data reduction by Principal Component Analysis (PCA) or by wavenumber selection using statistical tests or genetic algorithms. Pseudo-colour maps were reconstructed and compared to conventional histology procedures. High correlation was obtained between the prediction maps and the histology which proves the great potential of FTIR spectroscopy for the differential diagnosis of skin carcinomas.Keywords
This publication has 24 references indexed in Scilit:
- Combination of FTIRspectral imaging and chemometrics for tumour detection from paraffin-embedded biopsiesThe Analyst, 2007
- Basal cell carcinoma: biology, morphology and clinical implicationsLaboratory Investigation, 2006
- Non-melanoma skin cancer: what drives tumor development and progression?Carcinogenesis: Integrative Cancer Research, 2005
- Imaging of colorectal adenocarcinoma using FT-IR microspectroscopy and cluster analysisBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2004
- The epidemiology of skin cancerBritish Journal of Dermatology, 2002
- Carcinogenesis of basal cell carcinomas: genetics and molecular mechanismsBritish Journal of Dermatology, 2002
- Histologic Variants of Squamous Cell Carcinoma of the SkinCancer Control, 2001
- Infrared Spectra of Basal Cell Carcinomas are Distinct from Non-Tumor-Bearing Skin ComponentsJournal of Investigative Dermatology, 1999
- Pathologic diagnosis as the gold standardCancer, 1997
- Keratin expression in basal cell carcinomasBritish Journal of Dermatology, 1992