Cellulase-free xylanases fromBacillusand other microorganisms
Open Access
- 1 February 2000
- journal article
- review article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 183 (1), 1-7
- https://doi.org/10.1111/j.1574-6968.2000.tb08925.x
Abstract
Xylanases are used mainly in the pulp and paper industries for the pretreatment of Kraft pulp prior to bleaching to minimize use of chlorine, the conventional bleaching agent. This application has great potential as an environmentally safe method. Hydrolysis by xylanases of relocated and reprecipitated xylan on the surface of cellulose fibres formed during Kraft cooking facilitates the removal of lignin by increasing permeability to oxidising agents. Most of the xylanases reported in the literature contained significant cellulolytic activity, which make them less suitable for pulp and paper industries. The need for large quantities of xylanases which would be stable at higher temperatures and pH values and free of cellulase activity has necessitated a search for novel enzymes. We have isolated and characterised several xylanase-producing cultures, one of which (an alkalophilic Bacillus SSP-34) produced more than 100 IU ml−1 of xylanase activity. The SSP-34 xylanases have optimum activity at 50°C in a pH range 6–8, with only small amounts of cellulolytic activity (CMCase (0.4 IU ml−1, pH 7), FPase (0.2 IU ml−1, pH 7) and no activity at pH 9).Keywords
This publication has 56 references indexed in Scilit:
- Production of fungal xylanasesBioresource Technology, 1996
- Thermophilic Alkaline Xylanase from Newly Isolated Alkaliphilic and ThermophilicBacillussp. Strain TAR-1Bioscience, Biotechnology, and Biochemistry, 1994
- Thermostable xylanolytic enzymes from Rhodothermus marinus grown on xylanApplied Microbiology and Biotechnology, 1993
- Production of cellulolytic and xylanolytic enzymes by an Arthrographis speciesWorld Journal of Microbiology and Biotechnology, 1993
- Purification and properties of a β-1,4-xylanase from a cellulolytic extreme thermophile expressed in Escherichia coliInternational Journal of Biochemistry, 1993
- Delignification of wood pulp by a thermostable xylanase from Bacillus stearothermophilus strain T-6Biodegradation, 1992
- Origin and Nature of Kraft Colour: 1 Role of AromaticsJournal of Wood Chemistry and Technology, 1990
- Physiological Disturbances in Fish Exposed to Bleached Kraft Mill EffluentsWater Science & Technology, 1988
- Evaluation of different microbial xylanolytic systemsJournal of Biotechnology, 1987
- Reactions of D-xylose and D-glucose in alkaline, aqueous solutionsCarbohydrate Research, 1976