Ultradeep Pyrosequencing Detects Complex Patterns of CD8 + T-Lymphocyte Escape in Simian Immunodeficiency Virus-Infected Macaques
Open Access
- 15 August 2009
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 83 (16), 8247-8253
- https://doi.org/10.1128/jvi.00897-09
Abstract
Human and simian immunodeficiency viruses (HIV/SIV) exhibit enormous sequence heterogeneity within each infected host. Here, we use ultradeep pyrosequencing to create a comprehensive picture of CD8 + T-lymphocyte (CD8-TL) escape in SIV-infected macaques, revealing a previously undetected complex pattern of viral variants. This increased sensitivity enabled the detection of acute CD8-TL escape as early as 17 days postinfection, representing the earliest published example of CD8-TL escape in intrarectally infected macaques. These data demonstrate that pyrosequencing can be used to study the evolution of CD8-TL escape during immunodeficiency virus infection with an unprecedented degree of sensitivity.This publication has 24 references indexed in Scilit:
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