Electrochemical Surface Plasmon Resonance and Waveguide-Enhanced Glucose Biosensing with N-Alkylaminated Polypyrrole/Glucose Oxidase Multilayers
- 28 July 2010
- journal article
- research article
- Published by American Chemical Society (ACS) in ACS Applied Materials & Interfaces
- Vol. 2 (8), 2347-2354
- https://doi.org/10.1021/am100373v
Abstract
In this work, we report an electrochemical surface plasmon resonance/waveguide (EC-SPR/waveguide) glucose biosensor that could detect enzymatic reactions in a conducting polymer/glucose oxidase (GOx) multilayer thin film. In order to achieve a controlled enzyme electrode and waveguide mode, GOx (negatively charged) was immobilized with a water-soluble, conducting N-alkylaminated polypyrrole (positively charged) using the layer-by-layer (LbL) electrostatic self-assembly technique. The electrochemical and optical signals were simultaneously obtained from the composite LbL enzyme electrode upon the addition of glucose as mediated by the electroactivity and electrochromic property of the polypyrrole layers. Signal enhancement in EC-SPR detection is obtained by monitoring the doping−dedoping events on the polypyrrole. The real-time optical signal could be distinguished between the change in the dielectric constant of the enzyme layer and other nonenzymatic reaction events such as adsorption of glucose and the change of the refractive index of the solution. This was possible by correlation of both the SPR mode and the m = 0 and 1 modes of the waveguide in an SPR/waveguide spectroscopy experiment.Keywords
This publication has 29 references indexed in Scilit:
- Optical Waveguide Spectroscopy for the Investigation of Protein‐Functionalized Hydrogel FilmsMacromolecular Rapid Communications, 2009
- Layer-by-Layer Biosensor Assembly Incorporating Functionalized Quantum DotsLangmuir, 2003
- Chip and solution detection of DNA hybridization using a luminescent zwitterionic polythiophene derivativeNature Materials, 2003
- In Situ Analysis of Electropolymerization of Aniline by Combined Electrochemistry and Surface Plasmon ResonanceLangmuir, 2002
- In Situ Investigations on the Electrochemical Polymerization and Properties of Polyaniline Thin Films by Surface Plasmon Optical TechniquesThe Journal of Physical Chemistry B, 2002
- Photonic transduction of electrochemically-triggered redox-functions of polyaniline films using surface plasmon resonance spectroscopyChemical Communications, 2001
- Properties of glucose sensors based on the immobilization of glucose oxidase in N-substituted polypyrrole filmSensors and Actuators B: Chemical, 2000
- Nano-assembly of glucose oxidase on thein situself-assembled films of polypyrrole and its optical, surface and electrochemical characterizationsNanotechnology, 2000
- Properties of glucose sensors prepared by the electropolymerization of a positively charged pyrrole derivativeElectrochemistry Communications, 1999
- Protein-modified electrodes. The glucose oxidase/polypyrrole systemAnalytical Chemistry, 1986