A MicroRNA Superfamily Regulates Nucleotide Binding Site–Leucine-Rich Repeats and Other mRNAs
Top Cited Papers
- 9 March 2012
- journal article
- Published by Oxford University Press (OUP) in THE PLANT CELL ONLINE
- Vol. 24 (3), 859-874
- https://doi.org/10.1105/tpc.111.095380
Abstract
Analysis of tomato (Solanum lycopersicum) small RNA data sets revealed the presence of a regulatory cascade affecting disease resistance. The initiators of the cascade are microRNA members of an unusually diverse superfamily in which miR482 and miR2118 are prominent members. Members of this superfamily are variable in sequence and abundance in different species, but all variants target the coding sequence for the P-loop motif in the mRNA sequences for disease resistance proteins with nucleotide binding site (NBS) and leucine-rich repeat (LRR) motifs. We confirm, using transient expression in Nicotiana benthamiana, that miR482 targets mRNAs for NBS-LRR disease resistance proteins with coiled-coil domains at their N terminus. The targeting causes mRNA decay and production of secondary siRNAs in a manner that depends on RNA-dependent RNA polymerase 6. At least one of these secondary siRNAs targets other mRNAs of a defense-related protein. The miR482-mediated silencing cascade is suppressed in plants infected with viruses or bacteria so that expression of mRNAs with miR482 or secondary siRNA target sequences is increased. We propose that this process allows pathogen-inducible expression of NBS-LRR proteins and that it contributes to a novel layer of defense against pathogen attack.Keywords
This publication has 77 references indexed in Scilit:
- An Extensive MicroRNA-Mediated Network of RNA-RNA Interactions Regulates Established Oncogenic Pathways in GlioblastomaCell, 2011
- Coding-Independent Regulation of the Tumor Suppressor PTEN by Competing Endogenous mRNAsCell, 2011
- A Long Noncoding RNA Controls Muscle Differentiation by Functioning as a Competing Endogenous RNACell, 2011
- In Vivo Identification of Tumor-Suppressive PTEN ceRNAs in an Oncogenic BRAF-Induced Mouse Model of MelanomaCell, 2011
- Transcriptional Control of Gene Expression by MicroRNAsCell, 2010
- Origin, Biogenesis, and Activity of Plant MicroRNAsCell, 2009
- MicroRNAs: Target Recognition and Regulatory FunctionsCell, 2009
- The Tomato Sequencing Project, the First Cornerstone of the International Solanaceae Project (SOL)Comparative and Functional Genomics, 2005
- Genome-wide identification of NBS genes in japonica rice reveals significant expansion of divergent non-TIR NBS-LRR genesMolecular Genetics and Genomics, 2004
- Affinity Fusion Strategies for Detection, Purification, and Immobilization of Recombinant ProteinsProtein Expression and Purification, 1997