Use of an autologous reactionin vitroto assess contributions of T and B lymphocytes to immune hyperreactivity of atopics
- 1 March 1989
- journal article
- research article
- Published by Wiley in Clinical and Experimental Allergy
- Vol. 19 (2), 163-168
- https://doi.org/10.1111/j.1365-2222.1989.tb02359.x
Abstract
The in-vitro proliferation reaction of peripheral blood lymphocytes (measured by [3H]thymidine incorporation) to autologous pokeweed mitogen (PWM)-induced lymphoblasts (PWM-lymphoblast-stimulated autologous mixed leucocyte reaction, PWM.AMLR) was used as a measure of immune hyperreactivity for comparison of atopic with non-atopic individuals. Accordingly, 10/24 non-atopics responded in the PWM.AMLR, and 19/19 atopics reacting to inhaled allergens responded. Autologous stimulation was associated with release of mitogenic factors from the PWM-activated stimulating cells (2/15 non-atopics, 9/15 atopics). For non-atopics, stimulation delivered by staphylococcus A (SAC)-activated cells was similar to that delivered by PWM-induced cells, while in atopics, the SAC.AMLR was never more than 50% of the PWM.AMLR, indicating a possible T cell component. Separation by panning of the stimulation cells into lymphocyte subsets supported the notion that stimulation involved a cooperation between B and T4+ T cells. It is proposed that a positive PWM.AMLR is dependent upon an initial B cell activation followed by the PWM stimulus dependent upon a previous T cell activation, where atopics have more lymphocytes in an activated state than healthy non-atopics. Such a baseline priming may contribute to an innate sensitivity of atopics to environmental allergens.Keywords
This publication has 11 references indexed in Scilit:
- On the immune reaction to autologous human lymphoblasts: Evidence for the stimulation by activating factors rather than induction by autoantigensClinical Immunology and Immunopathology, 1987
- Allergy indices based on allergen dose-response curve in a randomly selected sample of schoolchildrenAllergy, 1987
- B-cell stimulatory factor 1 activates resting B cells.Proceedings of the National Academy of Sciences of the United States of America, 1985
- Activated B lymphocytes: Stimulators of an augmented autologous mixed leukocyte reactionCellular Immunology, 1985
- Lymphocyte Transformation Induced by Autologous CellsInternational Archives of Allergy and Immunology, 1985
- Autologous Mixed Lymphocyte Reaction in Man.Allergy, 1983
- Abnormal Histamine-Induced Suppressor-Cell Function in Atopic SubjectsNew England Journal of Medicine, 1982
- Suppressor T‐lymphocyte activity in wheezy children with and without treatment by hyposensitizationClinical and Experimental Allergy, 1981
- Predisposing factors and the development of reaginic allergy in infancyClinical and Experimental Allergy, 1976