Purpose: To determine the phenolic composition and antiproliferative activity of 16 different extracts (hexane, dichloromethane, methanol and water) obtained from Bellisperennis, Convolvulusgalaticus, Trifoliumpannonicum and Lysimachiavulgaris on human breast cancer (MCF-7) and human hepatocellular carcinoma (HepG2/C3A) cell lines. Methods: The aerial parts of the plants were successively extracted with hexane, dichloromethane, methanol and water using a Soxhlet apparatus. The phenolic content of the plants were determined by plants by high performance liquid chromatography (HPLC) while their antiproliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, a yellow tetrazole (MTT) assay. Results: Among the tested extracts, the methanol extract of B. perennis showed the best antiproliferative activity against MCF-7 cell line with IC50 (inhibiting 50 % of cell growth) value of 71.6 μg/mL. Furthermore, the dichloromethane extract of C. galaticus showed the best anti-proliferative activity against HepG2/C3A cell line with IC50 of 57.3 μg/mL. The HPLC data for the plant extracts showed the presence of the following phenolic compounds: gallic acid monohydrate, caffeic acid, rutin hydrate, luteolin-7-O-β-D glucoside, kaempferol, myricetin, quercetin, coumarin and apigenin. Conclusion: The findings of this study indicate that there is some justification for the use of B. perennis and C. galaticus as traditional anticancer medicinal herbs. Keywords: Bellis perennis, Convolvulus galaticus, Trifolium pannonicum subsp. elongatum, Lysimachia vulgaris, MCF-7, HepG2/C3A, Phenolics, Breast cancer, Antiproliferative