Novel In Vivo -Degradable Cellulose-Chitin Copolymer from Metabolically Engineered Gluconacetobacter xylinus
Open Access
- 15 September 2010
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 76 (18), 6257-6265
- https://doi.org/10.1128/aem.00698-10
Abstract
Despite excellent biocompatibility and mechanical properties, the poor in vitro and in vivo degradability of cellulose has limited its biomedical and biomass conversion applications. To address this issue, we report a metabolic engineering-based approach to the rational redesign of cellular metabolites to introduce N-acetylglucosamine (GlcNAc) residues into cellulosic biopolymers during de novo synthesis from Gluconacetobacter xylinus. The cellulose produced from these engineered cells (modified bacterial cellulose [MBC]) was evaluated and compared with cellulose produced from normal cells (bacterial cellulose [BC]). High GlcNAc content and lower crystallinity in MBC compared to BC make this a multifunctional bioengineered polymer susceptible to lysozyme, an enzyme widespread in the human body, and to rapid hydrolysis by cellulase, an enzyme commonly used in biomass conversion. Degradability in vivo was demonstrated in subcutaneous implants in mice, where modified cellulose was completely degraded within 20 days. We provide a new route toward the production of a family of tailorable modified cellulosic biopolymers that overcome the longstanding limitation associated with the poor degradability of cellulose for a wide range of potential applications.Keywords
This publication has 46 references indexed in Scilit:
- Engineering prokaryotic gene circuitsFEMS Microbiology Reviews, 2008
- Metabolic engineering of a thermophilic bacterium to produce ethanol at high yieldProceedings of the National Academy of Sciences, 2008
- Genomics of cellulosic biofuelsNature, 2008
- The Future Prospects of Microbial Cellulose in Biomedical ApplicationsBiomacromolecules, 2006
- Enhancement of Cellulose Pellicle Production by Constitutively Expressing Vitreoscilla Hemoglobin in Acetobacter xylinumBiotechnology Progress, 2006
- Enhancement of Cellulose Pellicle Production by Constitutively ExpressingVitreoscillaHemoglobin inAcetobacter xylinumBiotechnology Progress, 2006
- Current and Emerging Approaches for Natural Product Biosynthesis in Microbial CellsAdvanced Synthesis & Catalysis, 2005
- Direct Incorporation of Glucosamine and N -Acetylglucosamine into Exopolymers by Gluconacetobacter xylinus (= Acetobacter xylinum ) ATCC 10245: Production of Chitosan-Cellulose and Chitin-Cellulose ExopolymersApplied and Environmental Microbiology, 2001
- Structural changes of native cellulose crystals induced by annealing in aqueous alkaline and acidic solutions at high temperaturesMacromolecules, 1989
- The Structure of Regenerated CelluloseMacromolecules, 1975