Activation of the transcription factor nuclear factor‐κB in human inflamed synovial tissue
Open Access
- 1 April 1996
- journal article
- basic science
- Published by Wiley in Arthritis & Rheumatism
- Vol. 39 (4), 583-591
- https://doi.org/10.1002/art.1780390407
Abstract
Objective. The transcription factor–κB (NF-κB) has been implicated in the inflammatory response and is known to be activated by a process involving reactive oxygen intermediates. The purpose of the present study was to demonstrate the presence and distribution of activated NF-κB in synovium samples from patients with rheumatoid arthritis (RA) and osteoarthritis (OA) and from autopsy subjects with no known history of arthritis. Methods. Immunohistochemical staining was performed using both polyclonal and monoclonal “activity-specific” antibodies to the Rel-A (p65) subunit of NF-κB (anti-Rel-A nuclear location sequences). Histologic features of inflammation were also scored. Results. Both antibodies demonstrated positive staining of synovial tissue, with a cellular distribution that was nuclear. The staining was associated with specific cell types within the tissue, in particular, type A synoviocytes and vascular endothelium. Notably, lymphoid aggregates were unstained. Using the monoclonal antibody, a further study was carried out to investigate the distribution of staining in tissues from patients with different disease activities and clinical diagnoses, as well as in normal control tissue obtained at autopsy. Patients with acute RA more commonly showed vessel staining (P = 0.05) and, conversely, showed less frequent staining of the synovial lining (P < 0.005) compared with OA patients. Synovial tissue from controls exhibited either no staining or only weak staining in the synovial lining. Conclusion. The activation of NF-κB in vascular endothelium and type A synovial lining cells is a feature of synovial tissue from both RA and OA patients. The distribution of this staining appears to be related to the clinical diagnosis.Keywords
This publication has 34 references indexed in Scilit:
- Inhibition of the DNA‐binding activity of NF‐κB by gold compounds in vitroFEBS Letters, 1995
- Inhibition of the production and effects of interleukins‐1 and tumor necrosis factor α in rheumatoid arthritisArthritis & Rheumatism, 1995
- Expression of relB is required for the development of thymic medulla and dendritic cellsNature, 1995
- Expression of sequences homologous to htlv‐i tax gene in the labial salivary glands of japanese patients with sjögren's syndromeArthritis & Rheumatism, 1994
- Inhibition of NF-κB by Sodium Salicylate and AspirinScience, 1994
- Rapid proteolysis of IκB-α is necessary for activation of transcription factor NF-κBNature, 1993
- Presence of foam cells containing oxidised low density lipoprotein in the synovial membrane from patients with rheumatoid arthritis.Annals Of The Rheumatic Diseases, 1993
- Genetic control of inflammatory gene induction and NF-kappa B-like transcription factor activation in response to an atherogenic diet in mice.JCI Insight, 1993
- The american rheumatism association 1987 revised criteria for the classification of rheumatoid arthritisArthritis & Rheumatism, 1988
- Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.Journal of Histochemistry & Cytochemistry, 1981