tRNA cleavage is a conserved response to oxidative stress in eukaryotes
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- 21 August 2008
- journal article
- Published by Cold Spring Harbor Laboratory in RNA
- Vol. 14 (10), 2095-2103
- https://doi.org/10.1261/rna.1232808
Abstract
Recent results have identified a diversity of small RNAs in a wide range of organisms. In this work, we demonstrate that Saccharomyces cerevisiae contains a small RNA population consisting primarily of tRNA halves and rRNA fragments. Both 5′ and 3′ fragments of tRNAs are detectable by Northern blot analysis, suggesting a process of endonucleolytic cleavage. tRNA and rRNA fragment production in yeast is most pronounced during oxidative stress conditions, especially during entry into stationary phase. Similar tRNA fragments are also observed in human cell lines and in plants during oxidative stress. These results demonstrate that tRNA cleavage is a conserved aspect of the response to oxidative stress.Keywords
This publication has 40 references indexed in Scilit:
- Competition between the Rex1 exonuclease and the La protein affects both Trf4p-mediated RNA quality control and pre-tRNA maturationRNA, 2008
- Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5′–3′ exonucleases Rat1 and Xrn1Genes & Development, 2008
- Hidden layers of human small RNAsBMC Genomics, 2008
- RETRACTED: Elevated tRNAiMet Synthesis Can Drive Cell Proliferation and Oncogenic TransformationCell, 2008
- Small ncRNA transcriptome analysis from Aspergillus fumigatus suggests a novel mechanism for regulation of protein synthesisNucleic Acids Research, 2008
- Developmentally regulated cleavage of tRNAs in the bacterium Streptomyces coelicolorNucleic Acids Research, 2007
- The enigmatic effects of caffeine in cell cycle and cancerCancer Letters, 2007
- A New Yeast Poly(A) Polymerase Complex Involved in RNA Quality ControlPLoS Biology, 2005
- Human αA- and αB-crystallins bind to Bax and Bcl-XS to sequester their translocation during staurosporine-induced apoptosisCell Death & Differentiation, 2004
- tRNA breakdown products as markers for cancerCancer, 1979