Preferential digestion of basement membrane collagen by an enzyme derived from a metastatic murine tumor.
- 1 May 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (5), 2268-2272
- https://doi.org/10.1073/pnas.76.5.2268
Abstract
The specificity of human skin collagenase and of an enzyme from an invasive tumor were studied by using types I, II, III, IV and V (AB) collagen as substrates. Human skin collagenase degraded types I, II and III collagen, producing the characteristic 3/4 and 1/4 cleavage products, but failed to degrade type IV or V collagen. Collagenase prepared from the invasive tumor showed maximal activity after trypsin treatment. The tumor enzyme degraded type IV (basement membrane) collagen, producing fragments consistent with a single cleavage site but did not attack types I, II, III and V collagen. Because type IV collagen prepared by pepsinization of placenta was also digested, cleavage of type IV collagen by the tumor collagenase may occur within a largely helical domain. A type IV collagenase could play a significant role in tumor metastases and in normal tissues where basement membrane turnover takes place.Keywords
This publication has 24 references indexed in Scilit:
- Localization of The Collagenous Component in Skin Basement MembraneJournal of Investigative Dermatology, 1978
- Objective measurement of basement membrane abnormalities in human neoplasms of colorectum and of breastHistopathology, 1978
- Nature of the Collagenous Protein in a Tumor Basement MembraneEuropean Journal of Biochemistry, 1978
- Purification, characterization and inhibition of human skin collagenaseBiochemical Journal, 1978
- Degradation of Basement Membrane by Murine Tumor Cells2JNCI Journal of the National Cancer Institute, 1977
- Human skin collagenase: isolation of precursor and active forms from both fibroblast and organ culturesBiochemistry, 1977
- Granulocyte collagenase: selective digestion of type I relative to type III collagen.Proceedings of the National Academy of Sciences of the United States of America, 1977
- A murine tumor producing a matrix of basement membrane.The Journal of Experimental Medicine, 1977
- DISC ELECTROPHORESIS OF COLLAGEN COMPONENTSAnnals of the New York Academy of Sciences, 1964
- THE BEHAVIOR OF BASEMENT MEMBRANES IN INTRADUCTAL CARCINOMA OF THE BREAST1959