Mechanism of chimera formation during the Multiple Displacement Amplification reaction
Open Access
- 1 January 2007
- journal article
- Published by Springer Science and Business Media LLC in BMC Biotechnology
- Vol. 7 (1), 19
- https://doi.org/10.1186/1472-6750-7-19
Abstract
Multiple Displacement Amplification (MDA) is a method used for amplifying limiting DNA sources. The high molecular weight amplified DNA is ideal for DNA library construction. While this has enabled genomic sequencing from one or a few cells of unculturable microorganisms, the process is complicated by the tendency of MDA to generate chimeric DNA rearrangements in the amplified DNA. Determining the source of the DNA rearrangements would be an important step towards reducing or eliminating them.Keywords
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