Association of Intestinal Peptide Transport with a Protein Related to the Cadherin Superfamily
- 15 April 1994
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 264 (5157), 430-433
- https://doi.org/10.1126/science.8153632
Abstract
The first step in oral absorption of many medically important peptide-based drugs is mediated by an intestinal proton-dependent peptide transporter. This transporter facilitates the oral absorption of beta-lactam antibiotics and angiotensin-converting enzyme inhibitors from the intestine into enterocytes lining the luminal wall. A monoclonal antibody that blocked uptake of cephalexin was used to identify and clone a gene that encodes an approximately 92-kilodalton membrane protein that was associated with the acquisition of peptide transport activity by transport-deficient cells. The amino acid sequence deduced from the complementary DNA sequence of the cloned gene indicated that this transport-associated protein shares several conserved structural elements with the cadherin superfamily of calcium-dependent, cell-cell adhesion proteins.Keywords
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