Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves – GCMS based metabolomics approach
Open Access
- 1 September 2015
- journal article
- Published by The Journal of Food and Drug Analysis (JFDA), Food and Drug Administration, Taiwan (TFDA) in Journal of Food and Drug Analysis
- Vol. 23 (3), 433-441
- https://doi.org/10.1016/j.jfda.2015.01.005
Abstract
Cosmos caudatus, which is a commonly consumed vegetable in Malaysia, is locally known as “Ulam Raja”. It is a local Malaysian herb traditionally used as a food and medicinal herb to treat several maladies. Its bioactive or nutritional constituents consist of a wide range of metabolites, including glucosinolates, phenolics, amino acids, organic acids, and sugars. However, many of these metabolites are not stable and easily degraded or modified during storage. In order to investigate the metabolomics changes occurring during post-harvest storage, C. caudatus samples were subjected to seven different storage times (0 hours, 2 hours, 4 hours, 6 hours, 8 hours, 10 hours, and 12 hours) at room temperature. As the model experiment, the metabolites identified by gas chromatography-mass spectrometry (GC-MS) were correlated with α-glucosidase inhibitory activity analyzed with multivariate data analysis (MVDA) to find out the variation among samples and metabolites contributing to the activity. Orthogonal partial least squares (OPLS) analysis was applied to investigate the metabolomics changes. A profound chemical alteration, both in primary and secondary metabolites, was observed. The α-tocopherol, catechin, cyclohexen-1-carboxylic acid, benzoic acid, myo-inositol, stigmasterol, and lycopenecompoundswerefoundtobethediscriminatingmetabolitesatearlystorage;however, sugars such as sucrose, α-d-galactopyranose, and turanose were detected, which was attributed to the discriminating metabolites for late storage. The result shows that the MVDA method is a promising technique to identify biomarker compounds relative to storage at different times.Keywords
Funding Information
- Agro-Biotechnology Institute
- Ministry of Science, Technology and Innovation (6370007)
This publication has 40 references indexed in Scilit:
- The antioxidant and cytoprotective activity of Ocimum gratissimum extracts against hydrogen peroxide-induced toxicity in human HepG2 cellsJournal of Food and Drug Analysis, 2013
- Antidiabetic effects of natural plant extracts via inhibition of carbohydrate hydrolysis enzymes with emphasis on pancreatic alpha amylaseEmerging Therapeutic Targets, 2012
- Discrimination of young and mature leaves of Melicope ptelefolia using 1H NMR and multivariate data analysisFood Chemistry, 2011
- Inhibitory potential of ginger extracts against enzymes linked to type 2 diabetes, inflammation and induced oxidative stressInternational Journal of Food Sciences and Nutrition, 2010
- Effect of Extraction Solvent/Technique on the Antioxidant Activity of Selected Medicinal Plant ExtractsMolecules, 2009
- XCMS2: Processing Tandem Mass Spectrometry Data for Metabolite Identification and Structural CharacterizationAnalytical Chemistry, 2008
- Gas chromatography–mass spectrometry analysis of phenolic compounds from Carica papaya L. leafJournal of Food Composition and Analysis, 2007
- Antioxidant and nitric oxide inhibition activities of selected Malay traditional vegetablesFood Chemistry, 2006
- XCMS: Processing Mass Spectrometry Data for Metabolite Profiling Using Nonlinear Peak Alignment, Matching, and IdentificationAnalytical Chemistry, 2006
- Rapid screening and characterisation of antioxidants of Cosmos caudatus using liquid chromatography coupled with mass spectrometryJournal of Chromatography B, 2005