Immune Responses and Protection against ExperimentalBrucella suisBiovar 1 Challenge in Nonvaccinated orB. abortusStrain RB51-Vaccinated Cattle
- 1 December 2010
- journal article
- Published by American Society for Microbiology in Clinical and Vaccine Immunology
- Vol. 17 (12), 1891-1895
- https://doi.org/10.1128/cvi.00326-10
Abstract
Twenty Hereford heifers approximately 9 months of age were vaccinated with saline (control) or 2 × 10(10) CFU of the Brucella abortus strain RB51 (RB51) vaccine. Immunologic responses after inoculation demonstrated significantly greater (P < 0.05) antibody and proliferative responses to RB51 antigens in cattle vaccinated with RB51 than in the controls. Pregnant cattle received a conjunctival challenge at approximately 6 months of gestation with 10(7) CFU of B. suis bv. 1 strains isolated from naturally infected cattle. The fluorescence polarization assay and the buffered acid plate agglutination test had the highest sensitivities in detecting B. suis-infected cattle between 2 and 12 weeks after experimental infection. Serologic responses and lymphocyte proliferative responses to B. suis antigens did not differ between control and RB51 vaccinees after experimental infection. No abortions occurred in cattle in either treatment group after challenge, although there appeared to be an increased incidence of retained placenta after parturition in both the control and the RB51 vaccination treatment groups. Our data suggest that the mammary gland is a preferred site for B. suis localization in cattle. Vaccination with RB51 did not reduce B. suis infection rates in maternal or fetal tissues. In conclusion, although B. suis is unlikely to cause abortions and fetal losses in cattle, our data suggest that RB51 vaccination will not protect cattle against B. suis infection after exposure.Keywords
This publication has 17 references indexed in Scilit:
- Physiology and Treatment of Retained Fetal Membranes in CattleJournal of Veterinary Internal Medicine, 2010
- Immune Responses and Protection against Experimental Challenge after Vaccination of Bison withBrucella abortusStrain RB51 or RB51 Overexpressing Superoxide Dismutase and Glycosyltransferase GenesClinical and Vaccine Immunology, 2009
- Brucellaisolated in humans and animals in Latin America from 1968 to 2006Epidemiology and Infection, 2007
- Validation of FPA and cELISA for the detection of antibodies to Brucella abortus in cattle sera and comparison to SAT, CFT, and iELISAJournal of Immunological Methods, 2003
- Responses of adult cattle to vaccination with a reduced dose of Brucella abortus strain RB51Research in Veterinary Science, 2000
- Brucella SuisBiovar 1 in Naturally Infected Cattle: A Bacteriological, Serological, and Histological StudyJournal of Veterinary Diagnostic Investigation, 1997
- Effects of age at vaccination on efficacy of Brucella abortus strain RB51 to protect cattle against brucellosisAmerican Journal of Veterinary Research, 1996
- The identity, distribution and epizootiological significance of brucella isolates in Australia, 1981 to 1985Australian Veterinary Journal, 1987
- Isolation of Brucella suis from cattleAustralian Veterinary Journal, 1984
- BRUCELLA SUIS INFECTION IN PREGNANT CATTLEAustralian Veterinary Journal, 1979