THE FIRST COMPONENT OF THE KININ-FORMING SYSTEM IN HUMAN AND RABBIT PLASMA
Open Access
- 1 October 1971
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 134 (4), 986-1004
- https://doi.org/10.1084/jem.134.4.986
Abstract
The isolation and characterization of the first component of the kinin-forming system in human and rabbit plasma are presented. Functionally, the molecule is the precursor of the activator of prekallikrein (Pre-PKA) and evidence is presented that it is identical with Hageman factor (clotting factor XII). The component from each plasma possessed similar characteristics. This molecule was found to have a mol wt of 110,000 and sedimentation rate of 4.6S. It migrated in electrophoresis as a ß-globulin, having an isoelectric point of 6.1. Upon activation with glass, kaolin, diatomaceous earth, ellagic acid, or trypsin, the activated molecule converted purified prekallikrein (prokininogenase) to the active enzyme.Keywords
This publication has 16 references indexed in Scilit:
- A PREALBUMIN ACTIVATOR OF PREKALLIKREINThe Journal of Experimental Medicine, 1971
- A Pre-Albumin Activator of PrekallikreinThe Journal of Immunology, 1970
- Studies on the prekallikrein (kallikreinogen)—kallikrein enzyme system of human plasmaJCI Insight, 1969
- Partial purification of bovine plasma kallikreinogen, its activation by the Hageman factorBiochemical and Biophysical Research Communications, 1968
- Contact Activation of Hageman Factor and the Interaction of Hageman Factor and Plasma Thromboplastin AntecedentThrombosis and Haemostasis, 1967
- A Method of Trace Iodination of Proteins for Immunologic StudiesInternational Archives of Allergy and Immunology, 1966
- Hageman Factor: Alterations in Physical Properties during ActivationScience, 1965
- The gel-filtration behaviour of proteins related to their molecular weights over a wide rangeBiochemical Journal, 1965
- Activation of plasma by contact with glass: evidence for a common reaction which releases plasma kinin and initiates coagulationThe Journal of Physiology, 1958
- Plasma Pain-Producing Substance and Blood ClottingNature, 1957