Alterations in peripheral blood lymphocyte cytokine expression in obesity
Open Access
- 25 August 2006
- journal article
- Published by Oxford University Press (OUP) in Clinical and Experimental Immunology
- Vol. 146 (1), 39-46
- https://doi.org/10.1111/j.1365-2249.2006.03186.x
Abstract
Summary: Obesity is characterized by alterations in immune and inflammatory function. In order to evaluate the potential role of cytokine expression by peripheral blood mononuclear cells (PBMC) in obesity-associated inflammation, we studied serum protein levels and mRNA levels in PBMC of interleukin (IL)-6, IL-1β, tumour necrosis factor (TNF)-α and IL-1Ra in nine lean and 10 obese subjects. Serum IL-1β was undetectable, IL-1Ra serum levels were elevated, serum levels of TNF-α were decreased and serum levels of IL-6 were similar in obese subjects compared to lean subjects, while transcript levels of IL-6, IL-1β and TNF-α, but not IL-1Ra, were decreased in PBMC from obese subjects. PBMC from obese subjects did, however, up-regulate cytokine expression in response to leptin. Thus, obesity-associated changes in IL-1Ra serum levels and IL-6 mRNA levels were not correlated with changes in cognate mRNA and serum levels, respectively, while TNF-α serum levels and PBMC mRNA levels were both decreased in obese patients. While immune alterations in obesity are manifest in peripheral blood lymphocytes, the general lack of correlation between altered serum levels and altered PBMC gene expression suggests that PBMC may not be the source of aberrant serum cytokine levels in obesity.Keywords
This publication has 53 references indexed in Scilit:
- High-fat diet induces increased tissue expression of TNF-αLife Sciences, 2005
- Physiological regulation of hypothalamic IL-1β gene expression by leptin and glucocorticoids: implications for energy homeostasisAmerican Journal of Physiology-Endocrinology and Metabolism, 2004
- Hypothalamic Leptin Receptor and Signaling Molecule Expressions in Cafeteria Diet-Fed RatsJournal of Pharmacology and Experimental Therapeutics, 2003
- Overweight, Obesity, and Mortality from Cancer in a Prospectively Studied Cohort of U.S. AdultsNew England Journal of Medicine, 2003
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- TNF-α, leptin, and lymphocyte function in human agingLife Sciences, 2000
- Increased Soluble Tumor Necrosis Factor Receptor Levels in the Serum of Elderly PeopleGerontology, 2000
- Human Leptin Deficiency Caused by a Missense Mutation: Multiple Endocrine Defects, Decreased Sympathetic Tone, and Immune System Dysfunction Indicate New Targets for Leptin Action, Greater Central than Peripheral Resistance to the Effects of Leptin, and Spontaneous Correction of Leptin-Mediated DefectsJournal of Clinical Endocrinology & Metabolism, 1999
- INTERLEUKIN-1 RECEPTOR ANTAGONIST: Role in BiologyAnnual Review of Immunology, 1998
- A role for leptin and its cognate receptor in hematopoiesisCurrent Biology, 1996