Analysis of in vitro secretion profiles from adipose-derived cell populations
Open Access
- 22 August 2012
- journal article
- Published by Springer Science and Business Media LLC in Journal of Translational Medicine
- Vol. 10 (1), 172
- https://doi.org/10.1186/1479-5876-10-172
Abstract
Adipose tissue is an attractive source of cells for therapeutic purposes because of the ease of harvest and the high frequency of mesenchymal stem cells (MSCs). Whilst it is clear that MSCs have significant therapeutic potential via their ability to secrete immuno-modulatory and trophic cytokines, the therapeutic use of mixed cell populations from the adipose stromal vascular fraction (SVF) is becoming increasingly common. In this study we have measured a panel of 27 cytokines and growth factors secreted by various combinations of human adipose-derived cell populations. These were 1. co-culture of freshly isolated SVF with adipocytes, 2. freshly isolated SVF cultured alone, 3. freshly isolated adipocytes alone and 4. adherent adipose-derived mesenchymal stem cells (ADSCs) at passage 2. In addition, we produced an ‘in silico’ dataset by combining the individual secretion profiles obtained from culturing the SVF with that of the adipocytes. This was compared to the secretion profile of co-cultured SVF and adipocytes. Two-tailed t-tests were performed on the secretion profiles obtained from the SVF, adipocytes, ADSCs and the ‘in silico’ dataset and compared to the secretion profiles obtained from the co-culture of the SVF with adipocytes. A p-value of < 0.05 was considered statistically different. To assess the overall changes that may occur as a result of co-culture we compared the proteomes of SVF and SVF co-cultured with adipocytes using iTRAQ quantitative mass spectrometry. A co-culture of SVF and adipocytes results in a distinct secretion profile when compared to all other adipose-derived cell populations studied. This illustrates that cellular crosstalk during co-culture of the SVF with adipocytes modulates the production of cytokines by one or more cell types. No biologically relevant differences were detected in the proteomes of SVF cultured alone or co-cultured with adipocytes. The use of mixed adipose cell populations does not appear to induce cellular stress and results in enhanced secretion profiles. Given the importance of secreted cytokines in cell therapy, the use of a mixed cell population such as the SVF with adipocytes may be considered as an alternative to MSCs or fresh SVF alone.Keywords
This publication has 49 references indexed in Scilit:
- New SNP of the porcine Perilipin 2 (PLIN2) gene, association with carcass traits and expression analysis in skeletal muscleMolecular Biology Reports, 2010
- CD8+ effector T cells contribute to macrophage recruitment and adipose tissue inflammation in obesityNature Medicine, 2009
- Regulation of carboxylesterase 1 (CES1) in human adipose tissueBiochemical and Biophysical Research Communications, 2009
- Vasculotropic, paracrine actions of infused mesenchymal stem cells are important to the recovery from acute kidney injuryAmerican Journal of Physiology-Renal Physiology, 2007
- Macrophage-secreted factors induce adipocyte inflammation and insulin resistanceBiochemical and Biophysical Research Communications, 2006
- A Paracrine Loop Between Adipocytes and Macrophages Aggravates Inflammatory ChangesArteriosclerosis, Thrombosis, and Vascular Biology, 2005
- Human mesenchymal stem cells modulate allogeneic immune cell responsesBlood, 2005
- Mesenchymal stem cell engraftment in lung is enhanced in response to bleomycin exposure and ameliorates its fibrotic effectsProceedings of the National Academy of Sciences of the United States of America, 2003
- Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivoExperimental Hematology, 2002
- Effects of single-dose interleukin-12 exposure on interleukin-12-associated toxicity and interferon-gamma production.1997