Instability of IS6110 patterns in multidrug-resistant strains of Mycobacterium tuberculosis
- 26 June 2006
- journal article
- Published by Cambridge University Press (CUP) in Epidemiology and Infection
- Vol. 135 (2), 346-352
- https://doi.org/10.1017/s0950268806006790
Abstract
The stability of IS6110 restriction fragment length polymorphism (RFLP) pattern was determined in 31 isolates from patients with multidrug-resistant tuberculosis (MDR-TB). These patients were in actual chains of transmission and they referred to the National Institute of Tuberculosis and Lung Diseases, Tehran, Iran. Susceptibility testing against first- and second-line drugs were performed by the proportional method on Lowenstein–Jensen culture media. Thereafter, DNA fingerprinting by IS6110 with direct repeat (DR) region as a probe was performed by standard protocols. The rate of IS6110 changes was 16%, although, no variation was found in the DR region, in a time-span of 1–63 months. The strains with unstable IS6110 patterns were resistant to all drugs tested, and the majority of them (60%) were collected from HIV-positive patients. The results demonstrated that for a reliable interpretation of strain typing, it is better to use an additional marker along with IS6110 RFLP.Keywords
This publication has 13 references indexed in Scilit:
- Snapshot of Moving and Expanding Clones of Mycobacterium tuberculosis and Their Global Distribution Assessed by Spoligotyping in an International StudyJournal of Clinical Microbiology, 2003
- Global Distribution of Mycobacterium tuberculosis SpoligotypesEmerging Infectious Diseases, 2002
- Analysis of Rate of Change of IS6110RFLP Patterns ofMycobacterium tuberculosisBased on Serial Patient IsolatesThe Journal of Infectious Diseases, 1999
- Patterns of tuberculosis transmission in Central Los Angeles.JAMA, 1997
- Nosocomial Spread of Human Immunodeficiency Virus‐Related Multidrug‐Resistant Tuberculosis in Buenos AiresThe Journal of Infectious Diseases, 1997
- Origin and Interstate Spread of a New York City Multidrug-Resistant Mycobacterium tuberculosis Clone FamilyJAMA, 1996
- Use of IS6110 DNA fingerprinting in tracing man-to-man transmission of Mycobacterium tuberculosis in the Czech Republic.1996
- Transmission of Tuberculosis in New York City -- An Analysis by DNA Fingerprinting and Conventional Epidemiologic MethodsThe New England Journal of Medicine, 1994
- The Epidemiology of Tuberculosis in San Francisco -- A Population-Based Study Using Conventional and Molecular MethodsThe New England Journal of Medicine, 1994
- [15] DNA Fingerprinting of mycobacterium tuberculosisMethods in Enzymology, 1994