Amplification of pulsatile glucagon counterregulation by switch-off of α-cell-suppressing signals in streptozotocin-treated rats
- 1 September 2008
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Endocrinology and Metabolism
- Vol. 295 (3), E575-E585
- https://doi.org/10.1152/ajpendo.90372.2008
Abstract
Glucagon counterregulation (GCR) is a key protection against hypoglycemia that is compromised in diabetes via an unknown mechanism. To test the hypothesis that α-cell-inhibiting signals that are switched off during hypoglycemia amplify GCR, we studied streptozotocin (STZ)-treated male Wistar rats and estimated the effect on GCR of intrapancreatic infusion and termination during hypoglycemia of saline, insulin, and somatostatin. Times 10 min before and 45 min after the switch-off were analyzed. Insulin and somatostatin, but not saline, switch-off significantly increased the glucagon levels ( P = 0.03), and the fold increases relative to baseline were significantly higher ( P < 0.05) in the insulin and somatostatin groups vs. the saline group. The peak concentrations were also higher in the insulin (368 pg/ml) and somatostatin (228 pg/ml) groups vs. the saline (114 pg/ml) group ( P < 0.05). GCR was pulsatile in most animals, indicating a feedback regulation. After the switch-off, the number of secretory events and the total pulsatile production were lower in the saline group vs. the insulin and somatostatin groups ( P < 0.05), indicating enhancement of glucagon pulsatile activity by insulin and somatostatin compared with saline. Network modeling analysis demonstrates that reciprocal interactions between α- and δ-cells can explain the amplification by interpreting the GCR as a rebound response to the switch-off. The model justifies experimental designs to further study the intrapancreatic network in relation to the switch-off phenomenon. The results of this proof-of-concept interdisciplinary study support the hypothesis that GCR develops as a rebound pulsatile response of the intrapancreatic endocrine feedback network to switch-off of α-cell-inhibiting islet signals.Keywords
This publication has 98 references indexed in Scilit:
- Chapter 15 AutoDeconMethods in Enzymology, 2009
- Glucokinase Mutations, Insulin Secretion, and Diabetes MellitusAnnual Review of Physiology, 1996
- Evidence that the brain of the conscious dog is insulin sensitive.JCI Insight, 1995
- Splanchnic Neural Regulation of Somatostatin Secretion in the Isolated Perfused Human PancreasAnnals of Surgery, 1994
- Effect of Muscarinic and Nicotinic-Cholinergic Blockade on the Glucagon Response to Insulin-Induced Hypoglycemia in Normal MenHormone and Metabolic Research, 1989
- Glucagon Responses to Hypoglycemia in Children and Adolescents With IDDMDiabetes Care, 1988
- Characteristics of Insulin and Glucagon Release from the Perfused Pancreas, Intact Isolated Islets, and Dispersed Islet CellsHormone Research, 1986
- Determination of immunoreactive somatostatin in rat plasma and responses to arginine, glucose and glucagon infusionDiabetologia, 1979
- Effects of glucose and arginine on the release of immunoreactive somatostatin from the isolated perfused rat pancreasFEBS Letters, 1978
- Lack of Glucagon Response to Hypoglycemia in Diabetes: Evidence for an Intrinsic Pancreatic Alpha Cell DefectScience, 1973