VP7 Gene of human rotavirus A genotype G5: Phylogenetic analysis reveals the existence of three different lineages worldwide
- 22 December 2010
- journal article
- research article
- Published by Wiley in Journal of Medical Virology
- Vol. 83 (2), 357-366
- https://doi.org/10.1002/jmv.21968
Abstract
Group A rotavirus (RV-A) genotype G5, which is common in pigs, was also detected in children with severe diarrhea in Brazil, Argentina, Paraguay, Cameroon, China, Thailand, and Vietnam. To evaluate the evolutionary relationship among RV-A G5 strains, the VP7 and VP4 genes of 28 Brazilian RV-A G5 human strains, sampled between 1986 and 2005, were sequenced and compared with other RV-A G5 strains currently circulating worldwide in animals and humans. The phylogenetic analysis of RV-A G5 VP7 gene strains demonstrates the existence of three main lineages: (a) Lineage I: Brazilian strains grouped with three porcine strains from Thailand; (b) Lineage II: porcine, bovine, and equine strains from different regions; (c) Lineage III: human strains isolated in Asia and Africa, and two porcine strains from Argentina. The VP8* (*non-typable) subunit of VP4 gene sequencing showed that all P[8] strains fell into three major genetic lineages: P[8]-1; P[8]-2; and P[8]-3. These results showed that the RV-A G5 strains circulating in humans are the result of two independent zoonotic transmission events, most likely from pigs. J. Med. Virol. 83:357–366, 2011.Keywords
This publication has 61 references indexed in Scilit:
- Identification of a G2-like porcine rotavirus bearing a novel VP4 type, P[32]Veterinary Research, 2010
- Sequence Analysis of Unusual P[7]G5 Bovine Rotavirus Strains Reveals Evidence of Interspecies TransmissionJournal of Clinical Microbiology, 2009
- Structure of Rotavirus Outer-Layer Protein VP7 Bound with a Neutralizing FabScience, 2009
- Characterization of novel VP7, VP4, and VP6 genotypes of a previously untypeable group A rotavirusVirology, 2009
- Recommendations for the classification of group A rotaviruses using all 11 genomic RNA segmentsArchiv für die gesamte Virusforschung, 2008
- Molecular Characterization of a New Variant of Rotavirus P[8]G9 Predominant in a Sentinel-Based Survey in Central ItalyJournal of Clinical Microbiology, 2007
- Identification of Group A Porcine Rotavirus Strains Bearing a Novel VP4 (P) Genotype in Italian Swine HerdsJournal of Clinical Microbiology, 2007
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- The rapid generation of mutation data matrices from protein sequencesBioinformatics, 1992
- A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequencesJournal of Molecular Evolution, 1980