In vitro culture of cynomolgus monkey embryos beyond early gastrulation
- 15 November 2019
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 366 (6467), 836-+
- https://doi.org/10.1126/science.aax7890
Abstract
Gastrulation is a key event in embryonic development when the germ layers are specified and the basic animal body plan is established. The complexities of primate gastrulation remain a mystery because of the difficulties in accessing primate embryos at this stage. Here, we report the establishment of an in vitro culture (IVC) system that supports the continuous development of cynomolgus monkey blastocysts beyond early gastrulation up to 20 days after fertilization. The IVC embryos highly recapitulated the key events of in vivo early postimplantation development, including segregation of the epiblast and hypoblast, formation of the amniotic and yolk sac cavities, appearance of the primordial germ cells, and establishment of the anterior-posterior axis. Single-cell RNA-sequencing analyses of the IVC embryos provide information about lineage specification during primate early postimplantation development. This system provides a platform with which to explore the characteristics and mechanisms of early postimplantation embryogenesis in primates with possible conservation of cell movements and lineages in human embryogenesis.Keywords
Funding Information
- National Natural Science Foundation of China (31590832)
- The National Natural Science Foundation of China (31571533)
- the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16020700)
- The National Key R&D Program of China (2018YFC1004500)
- The National Key R&D Program of China (2017YFC1001400)
- the exchange program of State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences (GREKF17-10)
This publication has 41 references indexed in Scilit:
- clusterProfiler: an R Package for Comparing Biological Themes Among Gene ClustersOMICS: A Journal of Integrative Biology, 2012
- Dynamics of anterior–posterior axis formation in the developing mouse embryoNature Communications, 2012
- Vimentin induces changes in cell shape, motility, and adhesion during the epithelial to mesenchymal transitionThe FASEB Journal, 2010
- Making a commitment: cell lineage allocation and axis patterning in the early mouse embryoNature Reviews Molecular Cell Biology, 2009
- Gene function in mouse embryogenesis: get set for gastrulationNature Reviews Genetics, 2007
- Roadmap to embryo implantation: clues from mouse modelsNature Reviews Genetics, 2006
- Culture of marmoset blastocysts on matrigel: A model of differentiation during the implantation periodThe Anatomical Record, 1995
- Differentiation of the embryonic disc, amnion, and yolk sac in the rhesus monkeyJournal of Anatomy, 1986
- Ultrastructure of mouse egg cylinders developed in vitroThe Anatomical Record, 1974
- In Vitro Support System for the Study of Blastocyst Differentiation in the MouseNature, 1970